A Whole-Genome Microarray Study of Arabidopsis thaliana Semisolid Callus Cultures Exposed to Microgravity and Nonmicrogravity Related Spaceflight Conditions for 5 Days on Board of Shenzhou 8

The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-15
Main Authors: Nieselt, Kay, Ecke, Margret, Neef, Maren, Spirer, Ina, Fengler, Svenja, Hampp, Rüdiger
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis thaliana
Biomedical research
Charged particles
Cluster Analysis
Culture
DNA sequencing
Experiments
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Plant
Gene Ontology
Genes
Genes, Plant
Genetic aspects
Genome, Plant
Genomes
Gravity
Kinases
Methods
Microgravity
Missions
Nucleotide sequencing
Observations
Oligonucleotide Array Sequence Analysis - methods
Onboard
Proteins
Semisolids
Signal transduction
Space Flight
Spacecraft
Studies
Temperature
Tissue Culture Techniques - methods
Weightlessness
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Summary:The Simbox mission was the first joint space project between Germany and China in November 2011. Eleven-day-old Arabidopsis thaliana wild type semisolid callus cultures were integrated into fully automated plant cultivation containers and exposed to spaceflight conditions within the Simbox hardware on board of the spacecraft Shenzhou 8. The related ground experiment was conducted under similar conditions. The use of an in-flight centrifuge provided a 1 g gravitational field in space. The cells were metabolically quenched after 5 days via RNAlater injection. The impact on the Arabidopsis transcriptome was investigated by means of whole-genome gene expression analysis. The results show a major impact of nonmicrogravity related spaceflight conditions. Genes that were significantly altered in transcript abundance are mainly involved in protein phosphorylation and MAPK cascade-related signaling processes, as well as in the cellular defense and stress responses. In contrast to short-term effects of microgravity (seconds, minutes), this mission identified only minor changes after 5 days of microgravity. These concerned genes coding for proteins involved in the plastid-associated translation machinery, mitochondrial electron transport, and energy production.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/547495