Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae

A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is...

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Bibliographic Details
Published in:Journal of clinical microbiology 2014-12, Vol.52 (12), p.4377-4380
Main Authors: Compain, Fabrice, Babosan, Ana, Brisse, Sylvain, Genel, Nathalie, Audo, Jennifer, Ailloud, Florent, Kassis-Chikhani, Najiby, Arlet, Guillaume, Decré, Dominique
Format: Article
Language:English
Subjects:
Antigens, Bacterial - genetics
Antigens, Surface - genetics
Bacteriological Techniques - methods
Bacteriology
Humans
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Molecular Epidemiology - methods
Multiplex Polymerase Chain Reaction - methods
Virulence Factors - genetics
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Summary:A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the surveillance of emerging highly virulent strains.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.02316-14