Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae

A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is...

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Published in:Journal of clinical microbiology 2014-12, Vol.52 (12), p.4377-4380
Main Authors: Compain, Fabrice, Babosan, Ana, Brisse, Sylvain, Genel, Nathalie, Audo, Jennifer, Ailloud, Florent, Kassis-Chikhani, Najiby, Arlet, Guillaume, Decré, Dominique
Format: Article
Language:English
Subjects:
Antigens, Bacterial - genetics
Antigens, Surface - genetics
Bacteriological Techniques - methods
Bacteriology
Humans
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Molecular Epidemiology - methods
Multiplex Polymerase Chain Reaction - methods
Virulence Factors - genetics
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container_end_page 4380
container_issue 12
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container_title Journal of clinical microbiology
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creator Compain, Fabrice
Babosan, Ana
Brisse, Sylvain
Genel, Nathalie
Audo, Jennifer
Ailloud, Florent
Kassis-Chikhani, Najiby
Arlet, Guillaume
Decré, Dominique
description A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the surveillance of emerging highly virulent strains.
doi_str_mv 10.1128/jcm.02316-14
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source PubMed (Medline); ASM_美国微生物学会期刊
subjects Antigens, Bacterial - genetics
Antigens, Surface - genetics
Bacteriological Techniques - methods
Bacteriology
Humans
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Molecular Epidemiology - methods
Multiplex Polymerase Chain Reaction - methods
Virulence Factors - genetics
title Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae
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