Enzyme immunoassay versus latex agglutination cryptococcal antigen assays in adults with non-HIV-related cryptococcosis

We compared paired enzyme immunoassay (EIA) and latex agglutination (LA) assay results with 185 blood and 164 cerebrospinal fluid (CSF) samples from 44 and 33 non-HIV cryptococcosis patients, respectively. The LA assay cutoff of 1:256 in the blood and 1:32 in the CSF was most highly predictive of a...

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Bibliographic Details
Published in:Journal of clinical microbiology 2014-12, Vol.52 (12), p.4356-4358
Main Authors: Panackal, Anil A, Dekker, John P, Proschan, Michael, Beri, Andrea, Williamson, Peter R
Format: Article
Language:English
Subjects:
Adult
Blood - microbiology
Cerebrospinal Fluid - microbiology
Cryptococcosis - diagnosis
Cryptococcus
Diagnostic Tests, Routine - methods
Humans
Immunoenzyme Techniques - methods
Latex Fixation Tests - methods
Mycology
Retrospective Studies
Sensitivity and Specificity
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Summary:We compared paired enzyme immunoassay (EIA) and latex agglutination (LA) assay results with 185 blood and 164 cerebrospinal fluid (CSF) samples from 44 and 33 non-HIV cryptococcosis patients, respectively. The LA assay cutoff of 1:256 in the blood and 1:32 in the CSF was most highly predictive of a positive EIA result. The EIA missed 18.4% detected by the LA assay in the blood samples and 7.8% detected by the LA assay in the CSF samples. We note here the improved sensitivity of the LA assay over the EIA in non-HIV patients.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.02017-14