Repair Tracts in Mismatched DNA Heteroduplexes

Heteroduplexes with mismatches at four sites were constructed from separated strands of λ DNA and used to transfect Escherichia coli under recombinationless conditions. The output phages from 967 single cells in one experiment and 1016 in another were analyzed to determine the pattern of mismatch re...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1976-11, Vol.73 (11), p.4135-4139
Main Authors: Wagner, Robert, Meselson, Matthew
Format: Article
Language:English
Subjects:
Bacteriophages
Base Sequence
Chromosome Mapping
Coefficients
Coliphages - metabolism
DNA
DNA mismatch repair
DNA Repair
DNA, Viral - metabolism
Event frequency
Frequency ranges
Genetics
Genotype
Genotypes
Molecular Weight
Nucleotides
Transfection
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Summary:Heteroduplexes with mismatches at four sites were constructed from separated strands of λ DNA and used to transfect Escherichia coli under recombinationless conditions. The output phages from 967 single cells in one experiment and 1016 in another were analyzed to determine the pattern of mismatch repair. A wide range of repair frequencies was found among the mismatches studied. Repair involving two or more close sites in the same heteroduplex occurs much more often on the same strand than on opposite strands. Analysis of the pattern of repair suggests that repair tracts initiate at mismatches, propagate preferentially in the 5′→ 3′ direction, and extend an average distance of ca 3000 nucleotides.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.73.11.4135