Peroxynitrite induced decrease in Na^＋, K^＋-ATPase activity is restored by taurine

• Published in: World journal of gastroenterology : WJG 2005-06, Vol.11 (23), p.3554-3557
• Main Author: NeclaKocak-Toker MuratGiris FetiTülübas MüjdatUysal GülcinAykac-Toker
• Format: Article
• Language: English
• Subjects: Animals; ATP酶; Brief Reports; Cell Membrane - drug effects; Cell Membrane - enzymology; Enzyme Inhibitors - pharmacology; Kinetics; Liver - enzymology; Mice; Oxidants - pharmacology; Peroxynitrous Acid - pharmacology; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors; Sodium-Potassium-Exchanging ATPase - metabolism; Taurine - pharmacology; 氧化剂; 肝脏损伤; 酶活性; 钠离子; 钾离子
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i23.3554

AIM: Peroxynitrite (ONOO^-) is a powerful oxidant shown to damage membranes. In the present study, the effect of taurine on changes of liver plasma membrane Na^+, K^+-ATPase induced by ONO0- was investigated. METHODS: Liver plasma membrane was exposed to ONOO^-with or without taurine. Na^+, K^+-ATPase activity and lipid peroxidation as thiobarbituric acid reactive substances (TBARS) levels were measured, RESULTS: Different concentrations of ONOO^- (100, 200, 500, and 1 000 μmol/L) were found to decrease liver plasma membrane Na^+, K^+-ATPase activity significantly. The depletion of enzyme activity was not concentration dependent. Effects of different concentrations of taurine on liver plasma membrane Na^+, K^+-ATPase activity were also measured. Taurine did not cause any increase in enzyme activity. When plasma membranes were treated with 200 μmol/L ONOO^- with different concentrations of taurine, a restoring effect of taurine on enzyme activity was observed. TBARS levels were also measured and taurine was found to decrease the elevated values. CONCLUSION: Taurine is observed to act as an antioxidant of ONOO^-to decrease lipid peroxidation and thus affect liver plasma membrane Na^+, K^+-ATPase by restoring its activity.