Inhibitory effect of adeno-associated virus-mediated gene transfer of human endostatin on hepatocellular carcinoma

AIM: To investigate the effect of adeno-associated virusmediated gene transfer of human endostatin on the growth of hepatocellular carcinoma (HCC).METHODS: HCC cell line Hep3B was infected with recombinant adeno-associated virus containing human endostatin gene (rAAV2-hEndo). The results of transfec...

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Published in:World journal of gastroenterology : WJG 2005-06, Vol.11 (22), p.3331-3334
Main Authors: Liu, Hong, Peng, Cheng-Hong, Liu, Ying-Bin, Wu, Yu-Lian, Zhao, Zhi-Ming, Wang, Yong, Han, Bao-San
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Carcinoma, Hepatocellular
Cell Line, Tumor
Endostatins - genetics
Gene Transfer Techniques
Humans
Liver Cancer
Liver Neoplasms
基因表达
病毒调节转换因子
细胞增殖
肝细胞癌
肿瘤细胞
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Summary:AIM: To investigate the effect of adeno-associated virusmediated gene transfer of human endostatin on the growth of hepatocellular carcinoma (HCC).METHODS: HCC cell line Hep3B was infected with recombinant adeno-associated virus containing human endostatin gene (rAAV2-hEndo). The results of transfection were detected by RT-PCR and SDS-PAGE assay. M-El assay was used to observe the effects of supernatant of transfected cells on ECV304 cell proliferation. An animal model of HCC was established by injecting Hep3B cells subcutaneously into the back of nude mice. Intratumoral injection of rAAV2-hEndo, empty virus and phosphate-buffered saline were given sequentially. Serum endostatin was determined by ELISA, the inhibitory effect of endostatin on the growth of xenograft was assessed in 3 wk.RESULTS: The results of RT-PCR and SDS-PAGE assay confirmed that rAAV2-hEndo successfully transfected Hep3B cells, and endostatin was secreted from Hep3B cells to medium. The supernatant of transfected cells markedly inhibited the proliferation of ECV304 cells(P
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v11.i22.3331