Adenovirus-mediated FasL gene transfer into human gastric carcinoma

• Published in: World journal of gastroenterology : WJG 2005-06, Vol.11 (22), p.3446-3450
• Main Authors: Zheng, Shi-Ying, Li, De-Chun, Zhang, Zhi-De, Zhao, Jun, Ge, Jin-Feng
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Animals; Brief Reports; Cell Division; Cell Line, Tumor; Fas Ligand Protein; Gene Transfer Techniques; Genetic Therapy - methods; Humans; Membrane Glycoproteins - genetics; Mice; Mice, Nude; Stomach Neoplasms - therapy; 基因表达; 病理机制; 腺病毒
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i22.3446

AIM: To evaluate the possible value of FasL in gastric cancer gene therapy by investigating the effects of FasL expression on human gastric cancer cell line.METHODS: An adenoviral vector encoding the full-length human FasL cDNA was constructed and used to infect a human gastric cancer (SGC-7901) cell line. FasL expression was confirmed by X-gal staining, flow cytometric analysis and RT-PCR. The effect of FasL on cell proliferation was determined by clonogenic assay, cytotoxicity was detectedby MTT assay, and cell viability was measured by trypan blue exclusion. The therapeutic efficiency of Ad-FasL in vivo was investigated with a xenograft tumor model in nude mice.RESULTS: SGC-7901 cells infected with Ad-FasL showed increased expression of FasL, resulting in significantly decreased cell growth and colony-forming activity when compared with control adenovirus-infected cells. The cytotoxicity of anti-Fas antibody (CH-11) in gastric cancer cells was stronger than that of ActD (91±8 vs60±5, P