Human bone marrow mesenchymal stem cell transplantation attenuates axonal injury in stroke rats

Previous studies have shown that transplantation of human bone marrow mesenchymal stem cells promotes neural functional recovery after stroke, but the neurorestorative mechanisms remain largely unknown. We hypothesized that functional recovery of myelinated axons may be one of underlying mechanisms....

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Bibliographic Details
Published in:Neural regeneration research 2014-12, Vol.9 (23), p.2053-2058
Main Authors: Xu, Yi, Du, Shiwei, Yu, Xinguang, Han, Xiao, Hou, Jincai, Guo, Hao
Format: Article
Language:English
Subjects:
Adhesives
Bone marrow
Brain
Carotid arteries
Degeneration
FDA approval
Genetic aspects
Growth factors
Ischemia
Laboratory animals
Nervous system
Polyclonal antibodies
Proteins
Research and Report
Stem cell transplantation
Stem cells
Stroke
Stroke (Disease)
Transplantation
Veins & arteries
中风
大鼠模型
干细胞移植
神经保护作用
衰减
轴突损伤
骨髓间充质干细胞
髓鞘碱性蛋白
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Summary:Previous studies have shown that transplantation of human bone marrow mesenchymal stem cells promotes neural functional recovery after stroke, but the neurorestorative mechanisms remain largely unknown. We hypothesized that functional recovery of myelinated axons may be one of underlying mechanisms. In this study, an ischemia/reperfusion rat model was established using the middle cerebral artery occlusion method. Rats were used to test the hypothesis that intravenous transplantation of human bone marrow mesenchyrnal stem cells through the femoral vein could exert neuroprotective effects against cerebral ischemia via a mechanism associated with the ability to attenuate axonal injury. The results of behavioral tests, infarction volume analysis and immunohistochemistry showed that cerebral ischemia caused severe damage to the myelin sheath and axons. After rats were intravenously transplanted with human bone marrow mesenchymal stem cells, the levels of axon and myelin sheath-related proteins, including microtubule-associated protein 2, myelin basic protein, and growth-associated protein 43, were elevated, infarct volume was decreased and neural function was improved in cerebral ischemic rats. These findings suggest that intravenously transplanted human bone marrow mesenchymal stem cells promote neural function. Possible mechanisms underlying these beneficial effects include resistance to demyelination after cerebral ischemia, prevention of axonal degeneration, and promotion of axonal regeneration.
ISSN:1673-5374
1876-7958
DOI:10.4103/1673-5374.147930