Lower esophageal microbiota species are affected by the eradication of Helicobacter pylori infection using antibiotics

The aim of this study was to investigate the effect of Helicobacter pylori (H. pylori) infection on the lower esophageal microbiota and the eradication of H. pylori through the use of antibiotics. Forty-five BALB/C mice were randomly divided into negative control, infection and treatment groups. The...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2015-03, Vol.9 (3), p.685-692
Main Authors: TIAN, ZHIYING, YANG, ZHIBANG, GAO, JIYE, ZHU, LILI, JIANG, RENJU, JIANG, YING
Format: Article
Language:English
Subjects:
Antibiotics
Bacteria
Biotechnology industry
Deoxyribonucleic acid
DNA
Dosage and administration
Esophagus
Gastroesophageal reflux
Gram-negative bacteria
Helicobacter infections
Helicobacter pylori
HIV
Human immunodeficiency virus
infection
Infections
lower esophagus
Medical research
Medicine, Experimental
microbiota
Microbiota (Symbiotic organisms)
Pathogens
Pharmaceuticals
Physiological aspects
Polymerase chain reaction
polymerase chain reaction-denaturing gradient gel electrophoresis
Stomach
Studies
Ulcers
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Summary:The aim of this study was to investigate the effect of Helicobacter pylori (H. pylori) infection on the lower esophageal microbiota and the eradication of H. pylori through the use of antibiotics. Forty-five BALB/C mice were randomly divided into negative control, infection and treatment groups. The mice were sacrificed and DNA was extracted from the lower esophageal microbiota. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was performed to determine the composition of the microbiota. Quantity One® 1-D Analysis Software was used for the analysis of the DGGE profiles. The different bands from the groups were amplified with 16S rDNA V6 region primers. DNA sequencing and Basic Local Alignment Search Tool analysis were performed for the identification of the bands. H. pylori colonization led to severe ulcers in the stomachs of the mice, and these ulcers were alleviated by antibiotic treatment. The infection group had an increased number of bacterial species in the stomach compared with the control and treatment groups. DGGE fingerprinting of the lower esophagus showed that there were significant differences in the number of bands (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2015.2169