Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses

The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained...

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Bibliographic Details
Published in:Annals of clinical microbiology and antimicrobials 2015-01, Vol.14 (1), p.6-6, Article 6
Main Authors: Da Silva, Jr, Roberto Moreira, Da Silva Neto, João Ricardo, Santos, Carla Silvana, Frickmann, Hagen, Poppert, Sven, Cruz, Kátia Santana, Koshikene, Daniela, De Souza, João Vicente Braga
Format: Article
Language:English
Subjects:
Adult
Blood - microbiology
DNA, Fungal - genetics
Female
Fluorescence in situ hybridization
Fungal infections
Fungi - classification
Fungi - genetics
Fungi - isolation & purification
Humans
In Situ Hybridization, Fluorescence - methods
Male
Microbiology
Mycoses - diagnosis
Mycoses - microbiology
Polymerase Chain Reaction
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Summary:The aim of this study was to evaluate the diagnostic performance of in-house FISH (fluorescence in situ hybridisation) procedures for the direct identification of invasive fungal infections in blood cultures and cerebrospinal fluid (CSF) samples and to compare these FISH results with those obtained using traditional microbiological techniques and PCR targeting of the ITS1 region of the rRNA gene. In total, 112 CSF samples and 30 positive blood cultures were investigated by microscopic examination, culture, PCR-RFLP and FISH. The sensitivity of FISH for fungal infections in CSF proved to be slightly better than that of conventional microscopy (India ink) under the experimental conditions, detecting 48 (instead of 46) infections in 112 samples. The discriminatory powers of traditional microbiology, PCR-RFLP and FISH for fungal bloodstream infections were equivalent, with the detection of 14 fungal infections in 30 samples. However, the mean times to diagnosis after the detection of microbial growth by automated blood culture systems were 5 hours, 20 hours and 6 days for FISH, PCR-RFLP and traditional microbiology, respectively. The results demonstrate that FISH is a valuable tool for the identification of invasive mycoses that can be implemented in the diagnostic routine of hospital laboratories.
ISSN:1476-0711
1476-0711
DOI:10.1186/s12941-015-0065-5