Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation

• Published in: Cellular and molecular life sciences : CMLS 2015-03, Vol.72 (5), p.983-997
• Main Authors: Shi, Zhen-Hua, Shi, Fang-Fang, Wang, Yue-Qi, Sheftel, Alex D, Nie, Guangjun, Zhao, Ya-Shuo, You, Lin-Hao, Gou, Yu-Jing, Duan, Xiang-Lin, Zhao, Bao-Lu, Xu, Hong-Meng, Li, Chun-Yan, Chang, Yan-Zhong
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Biochemistry; Biomedical and Life Sciences; Biomedicine; brain; Cell Biology; Cell cycle; Cell Cycle Proteins - metabolism; Cell growth; Cell Line, Tumor; Cell Proliferation; Cyclin D1 - metabolism; Cyclin E - metabolism; cyclin-dependent kinase; Cyclin-Dependent Kinase 2 - metabolism; Cyclin-dependent kinases; Drosophila; ferritin; Ferritins - genetics; Ferritins - metabolism; G1 Phase Cell Cycle Checkpoints; gene expression regulation; gene overexpression; homeostasis; Humans; Intracellular Signaling Peptides and Proteins - metabolism; Iron; Life Sciences; Male; Metabolism; Mice; Mice, Inbred BALB C; Mice, Nude; Mitochondria; Mitochondria - metabolism; neoplasm cells; Neuroblastoma - metabolism; Neuroblastoma - pathology; nucleotide sequences; proliferating cell nuclear antigen; Proto-Oncogene Proteins c-myc - metabolism; Research Article; Retinoblastoma Protein - metabolism; therapeutics; transferrin; Tumor Suppressor Protein p53 - metabolism; Tumors
• ISSN: 1420-682X; 1420-9071
• DOI: 10.1007/s00018-014-1730-0

Mitochondrial ferritin (FtMt) has a significant effect on the regulation of cytosolic and mitochondrial iron levels. However, because of the deficiency of iron regulatory elements (IRE) in FtMt’s gene sequence, the exact function of FtMt remains unclear. In the present study, we found that FtMt dramatically inhibited SH-SY5Y cell proliferation and tumor growth in nude mice. Interestingly, excess FtMt did not adversely affect the development of drosophila. Additionally, we found that the expression of FtMt in human normal brain tissue was significantly higher than that of neuroblastoma, but not higher than that of neurospongioma. However, the expression of transferrin receptor 1 is completely opposite. We therefore hypothesized that increased expression of FtMt may negatively affect the vitality of neuronal tumor cells. Therefore, we further investigated the underlying mechanisms of FtMt’s inhibitory effects on neuronal tumor cell proliferation. As expected, FtMt overexpression disturbed the iron homeostasis of tumor cells and significantly downregulated the expression of proliferating cell nuclear antigen. Moreover, FtMt affected cell cycle, causing G1/S arrest by modifying the expression of cyclinD1, cyclinE, Cdk2, Cdk4 and p21. Remarkably, FtMt strongly upregulated the expression of the tumor suppressors, p53 and N-myc downstream-regulated gene-1 (NDRG1), but dramatically decreased C-myc, N-myc and p-Rb levels. This study demonstrates for the first time a new role and mechanism for FtMt in the regulation of cell cycle. We thus propose FtMt as a new candidate target for inhibiting neuronal tumor cell proliferation. Appropriate regulation of FtMt expression may prevent tumor cell growth. Our study may provide a new strategy for neuronal cancer therapy.