Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation

Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathog...

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Bibliographic Details
Published in:Scientific reports 2015-02, Vol.5 (1), p.8398-8398, Article 8398
Main Authors: Das, Theerthankar, Kutty, Samuel K., Tavallaie, Roya, Ibugo, Amaye I., Panchompoo, Janjira, Sehar, Shama, Aldous, Leigh, Yeung, Amanda W. S., Thomas, Shane R., Kumar, Naresh, Gooding, J. Justin, Manefield, Mike
Format: Article
Language:English
Subjects:
140/131
631/326
631/92
Antibiotics
Antioxidants
Antioxidants - metabolism
Biofilms
Deoxyribonuclease I - metabolism
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - metabolism
DNA structure
Electron transfer
Electron Transport
Extracellular Space - metabolism
Humanities and Social Sciences
Intercalating agents
Metabolites
multidisciplinary
Nucleic acids
Oxidation-Reduction
Pathogens
Phenazine
Phenazines - metabolism
Polymers
Protein Binding
Pseudomonas aeruginosa
Pseudomonas aeruginosa - pathogenicity
Pseudomonas aeruginosa - physiology
Pyocyanin
Pyocyanine - chemistry
Pyocyanine - metabolism
Science
Thermodynamics
Virulence factors
Virulence Factors - metabolism
Viscosity
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Summary:Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathogens such as Pseudomonas aeruginosa and hence reduce mortality. Here we reveal that the extracellular redox active virulence factor pyocyanin produced by P. aeruginosa binds directly to the deoxyribose-phosphate backbone of DNA and intercalates with DNA nitrogenous base pair regions. Binding results in local perturbations of the DNA double helix structure and enhanced electron transfer along the nucleic acid polymer. Pyocyanin binding to DNA also increases DNA solution viscosity. In contrast, antioxidants interacting with DNA and pyocyanin decrease DNA solution viscosity. Biofilms deficient in pyocyanin production and biofilms lacking extracellular DNA show similar architecture indicating the interaction is important in P. aeruginosa biofilm formation.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep08398