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Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation
Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathog...
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| Published in: | Scientific reports 2015-02, Vol.5 (1), p.8398-8398, Article 8398 |
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| Main Authors: | , , , , , , , , , , , |
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| container_end_page | 8398 |
| container_issue | 1 |
| container_start_page | 8398 |
| container_title | Scientific reports |
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| creator | Das, Theerthankar Kutty, Samuel K. Tavallaie, Roya Ibugo, Amaye I. Panchompoo, Janjira Sehar, Shama Aldous, Leigh Yeung, Amanda W. S. Thomas, Shane R. Kumar, Naresh Gooding, J. Justin Manefield, Mike |
| description | Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathogens such as
Pseudomonas aeruginosa
and hence reduce mortality. Here we reveal that the extracellular redox active virulence factor pyocyanin produced by
P. aeruginosa
binds directly to the deoxyribose-phosphate backbone of DNA and intercalates with DNA nitrogenous base pair regions. Binding results in local perturbations of the DNA double helix structure and enhanced electron transfer along the nucleic acid polymer. Pyocyanin binding to DNA also increases DNA solution viscosity. In contrast, antioxidants interacting with DNA and pyocyanin decrease DNA solution viscosity. Biofilms deficient in pyocyanin production and biofilms lacking extracellular DNA show similar architecture indicating the interaction is important in
P. aeruginosa
biofilm formation. |
| doi_str_mv | 10.1038/srep08398 |
| format | article |
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Pseudomonas aeruginosa
and hence reduce mortality. Here we reveal that the extracellular redox active virulence factor pyocyanin produced by
P. aeruginosa
binds directly to the deoxyribose-phosphate backbone of DNA and intercalates with DNA nitrogenous base pair regions. Binding results in local perturbations of the DNA double helix structure and enhanced electron transfer along the nucleic acid polymer. Pyocyanin binding to DNA also increases DNA solution viscosity. In contrast, antioxidants interacting with DNA and pyocyanin decrease DNA solution viscosity. Biofilms deficient in pyocyanin production and biofilms lacking extracellular DNA show similar architecture indicating the interaction is important in
P. aeruginosa
biofilm formation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep08398</identifier><identifier>PMID: 25669133</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>140/131 ; 631/326 ; 631/92 ; Antibiotics ; Antioxidants ; Antioxidants - metabolism ; Biofilms ; Deoxyribonuclease I - metabolism ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; DNA - metabolism ; DNA structure ; Electron transfer ; Electron Transport ; Extracellular Space - metabolism ; Humanities and Social Sciences ; Intercalating agents ; Metabolites ; multidisciplinary ; Nucleic acids ; Oxidation-Reduction ; Pathogens ; Phenazine ; Phenazines - metabolism ; Polymers ; Protein Binding ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - pathogenicity ; Pseudomonas aeruginosa - physiology ; Pyocyanin ; Pyocyanine - chemistry ; Pyocyanine - metabolism ; Science ; Thermodynamics ; Virulence factors ; Virulence Factors - metabolism ; Viscosity</subject><ispartof>Scientific reports, 2015-02, Vol.5 (1), p.8398-8398, Article 8398</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Feb 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited. All rights reserved 2015 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-f2547aff70c226d6ca92d543be9140ec3713431a1f2743d1732d31112dcc22053</citedby><cites>FETCH-LOGICAL-c438t-f2547aff70c226d6ca92d543be9140ec3713431a1f2743d1732d31112dcc22053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1898108325/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1898108325?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25733,27903,27904,36991,36992,44569,53769,53771,74872</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25669133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Das, Theerthankar</creatorcontrib><creatorcontrib>Kutty, Samuel K.</creatorcontrib><creatorcontrib>Tavallaie, Roya</creatorcontrib><creatorcontrib>Ibugo, Amaye I.</creatorcontrib><creatorcontrib>Panchompoo, Janjira</creatorcontrib><creatorcontrib>Sehar, Shama</creatorcontrib><creatorcontrib>Aldous, Leigh</creatorcontrib><creatorcontrib>Yeung, Amanda W. S.</creatorcontrib><creatorcontrib>Thomas, Shane R.</creatorcontrib><creatorcontrib>Kumar, Naresh</creatorcontrib><creatorcontrib>Gooding, J. Justin</creatorcontrib><creatorcontrib>Manefield, Mike</creatorcontrib><title>Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathogens such as
Pseudomonas aeruginosa
and hence reduce mortality. Here we reveal that the extracellular redox active virulence factor pyocyanin produced by
P. aeruginosa
binds directly to the deoxyribose-phosphate backbone of DNA and intercalates with DNA nitrogenous base pair regions. Binding results in local perturbations of the DNA double helix structure and enhanced electron transfer along the nucleic acid polymer. Pyocyanin binding to DNA also increases DNA solution viscosity. In contrast, antioxidants interacting with DNA and pyocyanin decrease DNA solution viscosity. Biofilms deficient in pyocyanin production and biofilms lacking extracellular DNA show similar architecture indicating the interaction is important in
P. aeruginosa
biofilm formation.</description><subject>140/131</subject><subject>631/326</subject><subject>631/92</subject><subject>Antibiotics</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Biofilms</subject><subject>Deoxyribonuclease I - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>DNA structure</subject><subject>Electron transfer</subject><subject>Electron Transport</subject><subject>Extracellular Space - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Intercalating agents</subject><subject>Metabolites</subject><subject>multidisciplinary</subject><subject>Nucleic acids</subject><subject>Oxidation-Reduction</subject><subject>Pathogens</subject><subject>Phenazine</subject><subject>Phenazines - metabolism</subject><subject>Polymers</subject><subject>Protein Binding</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - pathogenicity</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>Pyocyanin</subject><subject>Pyocyanine - chemistry</subject><subject>Pyocyanine - metabolism</subject><subject>Science</subject><subject>Thermodynamics</subject><subject>Virulence factors</subject><subject>Virulence Factors - metabolism</subject><subject>Viscosity</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNplkV1rFTEQhoMottRe-Ack4I0KRzOZ7NeNUOonFO2FXoec7OQ0ZTc5TXaL9tc3y6mHo-YmgXnyzjvzMvYcxFsQ2L7Libaixa59xI6lUNVKopSPD95H7DTna1FOJTsF3VN2JKu67gDxmN1cXlEwdz4Qv_VpHihY4s7YKSa-9qH3YcOnyOnXlIylYZgHk_iHb2fcZ-7HbUyTCRN3hb7MNPdxjMFkbijNGx9iNkUkOj-MCzKaycfwjD1xZsh0-nCfsJ-fPv44_7K6-P756_nZxcoqbKeVk5VqjHONsFLWfW1NJ_tK4Zo6UIIsNoAKwYCTjcIeGpQ9AoDsbfkgKjxh73e623k9Um8plBEGvU1-NOm3jsbrvyvBX-lNvNUKJdZVWwRePQikeDNTnvTo87IDEyjOWUNdHAqEbun18h_0Os4plPE0tF0LJR-5UK93lE0xl9jc3gwIvWSp91kW9sWh-z35J7kCvNkBuZTChtJBy__U7gEePKoN</recordid><startdate>20150211</startdate><enddate>20150211</enddate><creator>Das, Theerthankar</creator><creator>Kutty, Samuel K.</creator><creator>Tavallaie, Roya</creator><creator>Ibugo, Amaye I.</creator><creator>Panchompoo, Janjira</creator><creator>Sehar, Shama</creator><creator>Aldous, Leigh</creator><creator>Yeung, Amanda W. 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S.</au><au>Thomas, Shane R.</au><au>Kumar, Naresh</au><au>Gooding, J. Justin</au><au>Manefield, Mike</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-02-11</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>8398</spage><epage>8398</epage><pages>8398-8398</pages><artnum>8398</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathogens such as
Pseudomonas aeruginosa
and hence reduce mortality. Here we reveal that the extracellular redox active virulence factor pyocyanin produced by
P. aeruginosa
binds directly to the deoxyribose-phosphate backbone of DNA and intercalates with DNA nitrogenous base pair regions. Binding results in local perturbations of the DNA double helix structure and enhanced electron transfer along the nucleic acid polymer. Pyocyanin binding to DNA also increases DNA solution viscosity. In contrast, antioxidants interacting with DNA and pyocyanin decrease DNA solution viscosity. Biofilms deficient in pyocyanin production and biofilms lacking extracellular DNA show similar architecture indicating the interaction is important in
P. aeruginosa
biofilm formation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>25669133</pmid><doi>10.1038/srep08398</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Scientific reports, 2015-02, Vol.5 (1), p.8398-8398, Article 8398 |
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| language | eng |
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| source | Publicly Available Content Database; PubMed Central; Free Full-Text Journals in Chemistry; Springer Nature - nature.com Journals - Fully Open Access |
| subjects | 140/131 631/326 631/92 Antibiotics Antioxidants Antioxidants - metabolism Biofilms Deoxyribonuclease I - metabolism Deoxyribonucleic acid DNA DNA - chemistry DNA - metabolism DNA structure Electron transfer Electron Transport Extracellular Space - metabolism Humanities and Social Sciences Intercalating agents Metabolites multidisciplinary Nucleic acids Oxidation-Reduction Pathogens Phenazine Phenazines - metabolism Polymers Protein Binding Pseudomonas aeruginosa Pseudomonas aeruginosa - pathogenicity Pseudomonas aeruginosa - physiology Pyocyanin Pyocyanine - chemistry Pyocyanine - metabolism Science Thermodynamics Virulence factors Virulence Factors - metabolism Viscosity |
| title | Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation |
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