A Complex Genome-MicroRNA Interplay in Human Mitochondria

Small noncoding regulatory RNA exist in wide spectrum of organisms ranging from prokaryote bacteria to humans. In human, a systematic search for noncoding RNA is mainly limited to the nuclear and cytosolic compartments. To investigate whether endogenous small regulatory RNA are present in cell organ...

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Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-13
Main Authors: Santosh P., Shinde, Bhadra, Utpal
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Base Sequence
Bioinformatics
Cells, Cultured
Computational Biology - methods
Deoxyribonucleic acid
Disease
DNA
Gene expression
Genome, Mitochondrial - genetics
Genomes
Genomics
Health aspects
HEK293 Cells
Humans
Methods
Mice
MicroRNA
MicroRNAs
MicroRNAs - genetics
Mitochondria
Mitochondria - genetics
Mitochondrial DNA
Molecular Sequence Data
Muscle, Skeletal - metabolism
Mutation
Plant mitochondria
Polypeptides
Pongo - genetics
Proteins
Rats
RNA sequencing
Software
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Summary:Small noncoding regulatory RNA exist in wide spectrum of organisms ranging from prokaryote bacteria to humans. In human, a systematic search for noncoding RNA is mainly limited to the nuclear and cytosolic compartments. To investigate whether endogenous small regulatory RNA are present in cell organelles, human mitochondrial genome was also explored for prediction of precursor microRNA (pre-miRNA) and mature miRNA (miRNA) sequences. Six novel miRNA were predicted from the organelle genome by bioinformatics analysis. The structures are conserved in other five mammals including chimp, orangutan, mouse, rat, and rhesus genome. Experimentally, six human miRNA are well accumulated or deposited in human mitochondria. Three of them are expressed less prominently in Northern analysis. To ascertain their presence in human skeletal muscles, total RNA was extracted from enriched mitochondria by an immunomagnetic method. The expression of six novel pre-miRNA and miRNA was confirmed by Northern blot analysis; however, low level of remaining miRNA was found by sensitive Northern analysis. Their presence is further confirmed by real time RT-PCR. The six miRNA find their multiple targets throughout the human genome in three different types of software. The luciferase assay was used to confirm that MT-RNR2 gene was the potential target of hsa-miR-mit3 and hsa-miR-mit4.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/206382