Effects of the global regulator CsrA on the BarA/UvrY two-component signaling system

The hybrid sensor kinase BarA and its cognate response regulator UvrY, members of the two-component signal transduction family, activate transcription of CsrB and CsrC noncoding RNAs. These two small RNAs act by sequestering the RNA binding protein CsrA, which posttranscriptionally regulates transla...

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Bibliographic Details
Published in:Journal of bacteriology 2015-03, Vol.197 (5), p.983-991
Main Authors: Camacho, Martha I, Alvarez, Adrian F, Chavez, Ricardo Gonzalez, Romeo, Tony, Merino, Enrique, Georgellis, Dimitris
Format: Article
Language:English
Subjects:
Bacterial proteins
Bacteriology
Binding sites
Enzyme kinetics
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene expression
Gene Expression Regulation, Bacterial
Membrane Proteins - genetics
Membrane Proteins - metabolism
Phosphotransferases - genetics
Phosphotransferases - metabolism
Repressor Proteins - genetics
Repressor Proteins - metabolism
Ribonucleic acid
RNA
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Signal Transduction
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:The hybrid sensor kinase BarA and its cognate response regulator UvrY, members of the two-component signal transduction family, activate transcription of CsrB and CsrC noncoding RNAs. These two small RNAs act by sequestering the RNA binding protein CsrA, which posttranscriptionally regulates translation and/or stability of its target mRNAs. Here, we provide evidence that CsrA positively affects, although indirectly, uvrY expression, at both the transcriptional and translational levels. We also demonstrate that CsrA is required for properly switching BarA from its phosphatase to its kinase activity. Thus, the existence of a feedback loop mechanism that involves the Csr and BarA/UvrY global regulatory systems is exposed.
ISSN:0021-9193
1098-5530
DOI:10.1128/JB.02325-14