Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice

• Published in: Psychopharmacology 2015-03, Vol.232 (5), p.931-942
• Main Authors: Budzynska, Barbara, Boguszewska-Czubara, Anna, Kruk-Slomka, Marta, Skalicka-Wozniak, Krystyna, Michalak, Agnieszka, Musik, Irena, Biala, Grazyna
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - drug therapy; Alzheimer's disease; Animals; Antioxidants; Biomedical and Life Sciences; Biomedicine; Brain - drug effects; Care and treatment; Cognition - drug effects; Disease Models, Animal; Dosage and administration; Furocoumarins - pharmacology; Furocoumarins - therapeutic use; Laboratory rats; Male; Memory - drug effects; Memory Disorders - chemically induced; Memory Disorders - drug therapy; Mice; Neurosciences; Original Investigation; Oxidative stress; Oxidative Stress - drug effects; Pharmacology/Toxicology; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Practice guidelines (Medicine); Psychiatry; Psychopharmacology; Risk factors; Rodents; Scopolamine; Scopolamine Hydrobromide
• ISSN: 0033-3158; 1432-2072
• DOI: 10.1007/s00213-014-3728-6

Rationale Imperatorin, a naturally occurring furanocoumarin, inactivates gamma-aminobutyric acid transaminase and inhibits acetylcholinesterase activity. Objectives The purpose of our experiment was to examine the influence of imperatorin on cognitive impairment and oxidative stress in the brain induced by scopolamine in male Swiss mice. Methods In the present studies, we used scopolamine-invoke memory deficit measured in passive avoidance (PA) paradigm as an animal model of Alzheimer disease (AD). Results Our finding revealed that imperatorin administered acutely at the doses of 5 and 10 mg/kg prior to the injection of scopolamine (1 mg/kg) improved memory acquisition and consolidation impaired by scopolamine. Furthermore, repeatable (7 days, twice daily) administration of the highest dose of imperatorin (10 mg/kg) significantly attenuated the effects of scopolamine on memory acquisition, whereas the doses of 5 and 10 mg/kg of this furanocoumarin were effective when memory consolidation was measured. Imperatorin, administered with scopolamine, increased antioxidant enzymes activity and decreased concentration of malondiamide, an indicator of lipid peroxidation level. Conclusions These results demonstrate that imperatorin may offer protection against scopolamine-induced memory impairments and possesses antioxidant properties, thus after further preclinical and clinical studies this compound may provide an interesting approach in pharmacotherapy, as well as prophylactics of AD.