Cytoskeletal forces during signaling activation in Jurkat T-cells

T-cells are critical for the adaptive immune response in the body. The binding of the T-cell receptor (TCR) with antigen on the surface of antigen-presenting cells leads to cell spreading and signaling activation. The underlying mechanism of signaling activation is not completely understood. Althoug...

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Bibliographic Details
Published in:Molecular biology of the cell 2015-02, Vol.26 (4), p.685-695
Main Authors: Hui, King Lam, Balagopalan, Lakshmi, Samelson, Lawrence E, Upadhyaya, Arpita
Format: Article
Language:English
Subjects:
Adaptive Immunity
Cytoskeleton - physiology
Cytoskeleton - ultrastructure
Humans
Jurkat Cells
Mechanotransduction, Cellular
Receptors, Antigen, T-Cell - metabolism
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Summary:T-cells are critical for the adaptive immune response in the body. The binding of the T-cell receptor (TCR) with antigen on the surface of antigen-presenting cells leads to cell spreading and signaling activation. The underlying mechanism of signaling activation is not completely understood. Although cytoskeletal forces have been implicated in this process, the contribution of different cytoskeletal components and their spatial organization are unknown. Here we use traction force microscopy to measure the forces exerted by Jurkat T-cells during TCR activation. Perturbation experiments reveal that these forces are largely due to actin assembly and dynamics, with myosin contractility contributing to the development of force but not its maintenance. We find that Jurkat T-cells are mechanosensitive, with cytoskeletal forces and signaling dynamics both sensitive to the stiffness of the substrate. Our results delineate the cytoskeletal contributions to interfacial forces exerted by T-cells during activation.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E14-03-0830