MicroRNA profiling of the intestine during hypothermic circulatory arrest in swine

• Published in: World journal of gastroenterology : WJG 2015-02, Vol.21 (7), p.2183-2190
• Main Authors: Lin, Wei-Bin, Liang, Meng-Ya, Chen, Guang-Xian, Yang, Xiao, Qin, Han, Yao, Jian-Ping, Feng, Kang-Ni, Wu, Zhong-Kai
• Format: Article
• Language: English
• Subjects: Animals; bypass; Animal; Disease Models, Animal; Gene Expression Profiling - methods; Genetic Markers; Heart Arrest, Induced - adverse effects; Hypothermia, Induced - adverse effects; injury; Cardiopulmonary; Intestinal Mucosa - blood supply; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Mesenteric Ischemia - etiology; Mesenteric Ischemia - genetics; Mesenteric Ischemia - metabolism; Mesenteric Ischemia - pathology; MicroRNAs - metabolism; Permeability; Randomized Controlled Trial; Real-Time Polymerase Chain Reaction; Reperfusion; Reperfusion Injury - etiology; Reperfusion Injury - genetics; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Swine
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v21.i7.2183

AIM:To perform a profiling analysis of changes in intestinal micro RNA(mi RNA)expression during hypothermic circulatory arrest(HCA).METHODS:A total of eight piglets were randomly divided into HCA and sham operation(SO)groups.Under general anesthesia,swine in the HCA group were subjected to hypothermic cardiopulmonary bypass at 24?℃followed by 80 min of circulatory arrest,and the reperfusion lasted for 180 min after cross-clamp removal.The counterparts in the SO group were only subjected to median sternotomy.Histopathological analysis was used to detect mucosal injury,and Pickand-Mix custom mi RNA real-time polymerase chain reaction(PCR)panels containing 306 unique primer sets were utilized to assay unpooled intestinal samples harvested from the two groups.RESULTS:The intestinal mucosa of the animals that were subjected to 24?℃HCA exhibited representative ischemic reperfusion injury of grade 2 or 3 according to the Chiu score.Such intestinal mucosal injuries,with the subepithelial space and epithelial layer lifting away from the lamina propria,were accompanied by shortened and irregular villi.On the contrary,the intestinal mucosa remained normal in the shamoperated animals.In total,twenty-five mi RNAs were differentially expressed between the two groups(15upregulated and 10 downregulated in the HCA group).Among these,eight mi RNAs(mi R-122,mi R-221-5p,mi R-31,mi R-421-5p,mi R-4333,mi R-499-3p,mi R-542and let-7d-3p)were significantly dysregulated(four higher and four lower).The expression of mi R-122 was significantly(5.37-fold)increased in the HCA group vs the SO group,indicating that it may play a key role inHCA-induced mucosal injury.CONCLUSION:Exposure to HCA caused intestinal mi RNA dysregulation and barrier dysfunction in swine.These altered mi RNAs might be related to the protection or destruction of the intestinal barrier.