Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: Potential role in cardioprotection

Abstract Nitric oxide is an important messenger in numerous biological processes, such as angiogenesis, hypoxic vasodilation, and cardioprotection. Although nitric oxide synthases (NOS) produce the bulk of NO, there is increasing interest in NOS independent generation of NO in vivo , particularly du...

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Published in:Journal of molecular and cellular cardiology 2009-08, Vol.47 (2), p.256-263
Main Authors: Lohr, Nicole L, Keszler, Agnes, Pratt, Phillip, Bienengraber, Martin, Warltier, David C, Hogg, Neil
Format: Article
Language:English
Subjects:
Animals
Cardioprotection
Cardiotonic Agents - metabolism
Cardiovascular
Electron Spin Resonance Spectroscopy
Free Radical Scavengers - pharmacology
Hemeproteins - metabolism
Hemodynamics - drug effects
Hemoglobins - metabolism
Infrared Rays
Light
Luminescent Measurements
Myocardial Reperfusion Injury - physiopathology
Myoglobin - metabolism
Nitric oxide
Nitric Oxide - metabolism
Nitrite
Nitrosyl hemoglobin
Nitrosyl myoglobin
Ozone - metabolism
Rabbits
Spectrum Analysis
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Summary:Abstract Nitric oxide is an important messenger in numerous biological processes, such as angiogenesis, hypoxic vasodilation, and cardioprotection. Although nitric oxide synthases (NOS) produce the bulk of NO, there is increasing interest in NOS independent generation of NO in vivo , particularly during hypoxia or anoxia, where low oxygen tensions limit NOS activity. Interventions that can increase NO bioavailability have significant therapeutic potential. The use of far red and near infrared light (R/NIR) can reduce infarct size, protect neurons from methanol toxicity, and stimulate angiogenesis. How R/NIR modulates these processes in vivo and in vitro is unknown, but it has been suggested that increases in NO levels are involved. In this study we examined if R/NIR light could facilitate the release of NO from nitrosyl heme proteins. In addition, we examined if R/NIR light could enhance the protective effects of nitrite on ischemia and reperfusion injury in the rabbit heart. We show both in purified systems and in myocardium that R/NIR light can decay nitrosyl hemes and release NO, and that this released NO may enhance the cardioprotective effects of nitrite. Thus, the photodissociation to NO and its synergistic effect with sodium nitrite may represent a noninvasive and site-specific means for increasing NO bioavailability.
ISSN:0022-2828
1095-8584
DOI:10.1016/j.yjmcc.2009.03.009