Studies on the active constituents in radix salviae miltiorrhizae and their protective effects on cerebral ischemia reperfusion injury and its mechanism

To extract, purify and identify the active constituents in ethanol extract of Radix Salviae Miltiorrhizae, and to analyze the protective effects of tanshinone IIA on cerebral ischemia-reperfusion injury in rats. Radix Salviae Miltiorrhizae was extracted by ultrasonic extraction, effective parts were...

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Published in:Pharmacognosy Magazine 2015-01, Vol.11 (41), p.69-73
Main Authors: Li, Yan-Hui, Wang, Feng-Yun, Feng, Chun-Qing, Yang, Xia-Feng
Format: Article
Language:English
Subjects:
Abdomen
Brain research
Care and treatment
Cerebral ischemia
Chemical properties
Cold
Drug dosages
Ethanol
Free radicals
Health aspects
Ischemia
Original
Pain
Reperfusion injury
Rodents
Salvia
Studies
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Wang, Feng-Yun
Feng, Chun-Qing
Yang, Xia-Feng
description To extract, purify and identify the active constituents in ethanol extract of Radix Salviae Miltiorrhizae, and to analyze the protective effects of tanshinone IIA on cerebral ischemia-reperfusion injury in rats. Radix Salviae Miltiorrhizae was extracted by ultrasonic extraction, effective parts were extracted by extraction method, compounds were isolated by preparative TLC and preparative HPLC, and structures of compounds were identified by (1)H NMR and (13)C NMR; the effects of tanshinone IIA on cerebral ischemia-reperfusion injury in rats were determined by establishing rat model of middle cerebral artery occlusion (MCAO). The experimental data show four compounds were isolated, namely tanshinone IIB, hydroxymethylene tanshinone, salvianolic acid B and 9"'-methyl lithospermate B. Tanshinone IIA could alleviate the symptoms of neurological deficit in rats, the neurological deficit alleviating effect became more obvious with the increase of dose; tanshinone IIA experimental groups could reduce the cerebral infarction size and brain water content in rats, different concentrations of tanshinone IIA could decrease the SOD content and increase the MDA content in the frontal and parietal cortices of ischemic hemisphere in the ischemia reperfusion group, the differences were statistically significant compared with the ischemia reperfusion group. Radix Salviae Miltiorrhizae has the protective effects on cerebral ischemia reperfusion injury in rats.
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Tanshinone IIA could alleviate the symptoms of neurological deficit in rats, the neurological deficit alleviating effect became more obvious with the increase of dose; tanshinone IIA experimental groups could reduce the cerebral infarction size and brain water content in rats, different concentrations of tanshinone IIA could decrease the SOD content and increase the MDA content in the frontal and parietal cortices of ischemic hemisphere in the ischemia reperfusion group, the differences were statistically significant compared with the ischemia reperfusion group. Radix Salviae Miltiorrhizae has the protective effects on cerebral ischemia reperfusion injury in rats.</description><identifier>ISSN: 0973-1296</identifier><identifier>EISSN: 0976-4062</identifier><identifier>DOI: 10.4103/0973-1296.149706</identifier><identifier>PMID: 25709213</identifier><language>eng</language><publisher>United States: Medknow Publications and Media Pvt. 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Tanshinone IIA could alleviate the symptoms of neurological deficit in rats, the neurological deficit alleviating effect became more obvious with the increase of dose; tanshinone IIA experimental groups could reduce the cerebral infarction size and brain water content in rats, different concentrations of tanshinone IIA could decrease the SOD content and increase the MDA content in the frontal and parietal cortices of ischemic hemisphere in the ischemia reperfusion group, the differences were statistically significant compared with the ischemia reperfusion group. Radix Salviae Miltiorrhizae has the protective effects on cerebral ischemia reperfusion injury in rats.</abstract><cop>United States</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>25709213</pmid><doi>10.4103/0973-1296.149706</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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subjects Abdomen
Brain research
Care and treatment
Cerebral ischemia
Chemical properties
Cold
Drug dosages
Ethanol
Free radicals
Health aspects
Ischemia
Original
Pain
Reperfusion injury
Rodents
Salvia
Studies
title Studies on the active constituents in radix salviae miltiorrhizae and their protective effects on cerebral ischemia reperfusion injury and its mechanism
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