Endothelin inhibits renin release from juxtaglomerular cells via endothelin receptors A and B via a transient receptor potential canonical‐mediated pathway

Renin is the rate‐limiting step in the production of angiotensin II: a critical element in the regulation of blood pressure and in the pathogenesis of hypertension. Renin release from the juxtaglomerular (JG) cell is stimulated by the second messenger cAMP and inhibited by increases in calcium (Ca)....

Full description

Saved in:
Bibliographic Details
Published in:Physiological reports 2014-12, Vol.2 (12), p.e12240-n/a
Main Author: Ortiz‐Capisano, M. Cecilia
Format: Article
Language:English
Subjects:
Adenosine
Angiotensin
Angiotensin II
Blood pressure
Calcium
Endoplasmic reticulum
endothelin
Endothelin ETB receptors
Endothelin receptors
Endothelins
ETA
ETB
Immunofluorescence
Isoforms
Kinases
Laboratory animals
Original Research
Physiology
Renin
transient receptor potential canonical
Transient receptor potential proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Renin is the rate‐limiting step in the production of angiotensin II: a critical element in the regulation of blood pressure and in the pathogenesis of hypertension. Renin release from the juxtaglomerular (JG) cell is stimulated by the second messenger cAMP and inhibited by increases in calcium (Ca). Endothelins (ETs) inhibit renin release in a Ca‐dependent manner. JG cells contain multiple isoforms of canonical transient receptor potential (TRPC) Ca‐permeable channels. The proposed hypothesis is that endothelin inhibits renin release by activating TRPC store‐operated Ca channels. RT‐PCR and immunofluorescence revealed expression of both ETA and ETB receptors in mouse JG cells. Incubation of primary cultures of JG cells with ET‐1 (10 nmol/L) decreased renin release by 28%. Addition of either an ETA or an ETB receptor blocker completely prevented the ET inhibition of renin release. Incubation with the TRPC blocker (SKF 96365, 50 μmol/L) completely reversed the Ca‐mediated inhibition of renin release by ETs. These results suggest that endothelin inhibits renin release from JG cells via both ETA and ETB receptors, which leads to the activation of TRPC store‐operated Ca channels. e12240 This manuscript reexamines the relationship between endothelin and renin secretion from the juxtaglomerular cells, demonstrates the presence of endothelin A and B receptors on the cells. Furthermore, it describes a novel TRPC channel linking activation of the ETA/ETB to the calcium‐mediated inhibition of renin.
ISSN:2051-817X
2051-817X
DOI:10.14814/phy2.12240