Role of the nuclease of nontypeable Haemophilus influenzae in dispersal of organisms from biofilms

• Published in: Infection and immunity 2015-03, Vol.83 (3), p.950-957
• Main Authors: Cho, Christine, Chande, Aroon, Gakhar, Lokesh, Bakaletz, Lauren O, Jurcisek, Joseph A, Ketterer, Margaret, Shao, Jian, Gotoh, Kenji, Foster, Eric, Hunt, Jason, O'Brien, Erin, Apicella, Michael A
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Bacterial Load; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biofilms - growth & development; Chinchilla; Deoxyribonucleases - genetics; Deoxyribonucleases - metabolism; DNA - metabolism; Ear, Middle - microbiology; Ear, Middle - pathology; Escherichia coli - genetics; Escherichia coli - metabolism; Extracellular Space - chemistry; Gene Expression; Haemophilus influenzae; Haemophilus influenzae - enzymology; Haemophilus influenzae - genetics; Haemophilus influenzae - growth & development; Humans; Molecular Pathogenesis; Molecular Sequence Data; Mutation; Otitis Media - microbiology; Otitis Media - pathology; Plankton - growth & development; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Rodentia; Sequence Homology, Amino Acid; Staphylococcus aureus; Staphylococcus aureus - chemistry; Staphylococcus aureus - enzymology
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.02601-14

Nontypeable Haemophilus influenzae (NTHI) forms biofilms in the middle ear during human infection. The biofilm matrix of NTHI contains extracellular DNA. We show that NTHI possesses a potent nuclease, which is a homolog of the thermonuclease of Staphylococcus aureus. Using a biofilm dispersal assay, studies showed a biofilm dispersal pattern in the parent strain, no evidence of dispersal in the nuclease mutant, and a partial return of dispersion in the complemented mutant. Quantitative PCR of mRNA from biofilms from a 24-h continuous flow system demonstrated a significantly increased expression of the nuclease from planktonic organisms compared to those in the biofilm phase of growth (P < 0.042). Microscopic analysis of biofilms grown in vitro showed that in the nuclease mutant the nucleic acid matrix was increased compared to the wild-type and complemented strains. Organisms were typically found in large aggregates, unlike the wild-type and complement biofilms in which the organisms were evenly dispersed throughout the biofilm. At 48 h, the majority of the organisms in the mutant biofilm were dead. The nuclease mutant formed a biofilm in the chinchilla model of otitis media and demonstrated a propensity to also form similar large aggregates of organisms. These studies indicate that NTHI nuclease is involved in biofilm remodeling and organism dispersal.