Rat bone marrow stem cells isolation and culture as a bone formative experimental system

Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bos...

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Bibliographic Details
Published in:Bosnian journal of basic medical sciences 2013-02, Vol.13 (1), p.27-30
Main Authors: Smajilagic, Amer, Aljicevic, Mufida, Redzic, Amira, Filipovic, Selma, Lagumdzija, Alena C
Format: Article
Language:English
Subjects:
Animals
Ascorbic Acid - pharmacology
Bone marrow
Bone Morphogenetic Protein 7 - pharmacology
Bone Regeneration
Cell Culture Techniques
Cell Differentiation
Cell Separation - methods
Mesenchymal Stromal Cells - cytology
Methods
Osteocytes - cytology
Physiological aspects
Rats
Stem cells
Tissue engineering
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Summary:Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bosnia and Herzegovina, of the isolation and differentiation rat bone marrow stromal cells in to the osteoblast cells lineages. Rat bone marrow stromal cells were isolated by method described by Maniatopulos using their plastic adherence capatibility. The cells obtained by plastic adherence were cultured and serially passaged in the osteoinductive medium to differentiate into the osteocytes. Bone marrow samples from rats long bones used for isolation of stromal cells (BMSCs). Under determinate culture conditions BMSCs were differentiated in osteogenic cell lines detected by Alizarin red staining three weeks after isolation. BMSCs as autologue cells model showed high osteogenetic potential and calcification capatibility in vitro. In future should be used as alternative method for bone transplantation in Regenerative Medicine.
ISSN:1512-8601
1840-4812
DOI:10.17305/bjbms.2013.2409