The Ino80 complex prevents invasion of euchromatin into silent chromatin

Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncod...

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Bibliographic Details
Published in:Genes & development 2015-02, Vol.29 (4), p.350-355
Main Authors: Xue, Yong, Van, Christopher, Pradhan, Suman K, Su, Trent, Gehrke, Jason, Kuryan, Benjamin G, Kitada, Tasuku, Vashisht, Ajay, Tran, Nancy, Wohlschlegel, James, Peterson, Craig L, Kurdistani, Siavash K, Carey, Michael F
Format: Article
Language:English
Subjects:
Binding Sites
Chromatin - genetics
Euchromatin - genetics
Euchromatin - metabolism
Gene Expression Regulation, Fungal
Gene Silencing
Histone-Lysine N-Methyltransferase - metabolism
Methylation
Nuclear Proteins - metabolism
Protein Binding
Research Communication
RNA Polymerase II - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
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Summary:Here we show that the Ino80 chromatin remodeling complex (Ino80C) directly prevents euchromatin from invading transcriptionally silent chromatin within intergenic regions and at the border of euchromatin and heterochromatin. Deletion of Ino80C subunits leads to increased H3K79 methylation and noncoding RNA polymerase II (Pol II) transcription centered at the Ino80C-binding sites. The effect of Ino80C is direct, as it blocks H3K79 methylation by Dot1 in vitro. Heterochromatin stimulates the binding of Ino80C in vitro and in vivo. Our data reveal that Ino80C serves as a general silencing complex that restricts transcription to gene units in euchromatin.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.256255.114