Identification and Whole-Genome Sequence Analysis of Tembusu Virus GX2013G, Isolated from a Cherry Valley Duckling in Southern China

A duck tembusu virus (DTMUV) was isolated from the brain of a Cherry Valley duckling that showed neurological signs by using a specific-pathogen-free chicken embryo. The isolate was named GX2013G (GenBank accession no. KM275941). The strain GX2013G was identified with reverse transcription-PCR (RT-P...

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Bibliographic Details
Published in:Genome announcements (Washington, DC) DC), 2015-02, Vol.3 (1)
Main Authors: Zeng, Tingting, Xie, Zhixun, Xie, Liji, Deng, Xianwen, Xie, Zhiqin, Huang, Li, Luo, Sisi, Huang, Jiaoling
Format: Article
Language:English
Subjects:
amino acids
brain
chickens
China
ducks
genome
molecular epidemiology
nucleotides
open reading frames
pathogenesis
polyproteins
reverse transcriptase polymerase chain reaction
sequence analysis
specific pathogen-free animals
Tembusu virus
virus replication
Viruses
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Summary:A duck tembusu virus (DTMUV) was isolated from the brain of a Cherry Valley duckling that showed neurological signs by using a specific-pathogen-free chicken embryo. The isolate was named GX2013G (GenBank accession no. KM275941). The strain GX2013G was identified with reverse transcription-PCR (RT-PCR), and the amplicon was sequenced. The genome that was obtained is 10,990 nucleotides in length and contains a single open reading frame encoding a putative polyprotein of 3,425 amino acids. This study will advance the understanding of the epidemiology and molecular characteristics of tembusu virus (TMUV) in Guangxi and further studies of the mechanisms of virus replication and pathogenesis.
ISSN:2169-8287
2169-8287
DOI:10.1128/genomeA.00007-15