Expression profiles of microRNAs after focal cerebral ischemia/reperfusion injury in rats

Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed...

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Bibliographic Details
Published in:Neural regeneration research 2012-04, Vol.7 (12), p.917-923
Main Authors: Zhai, Fengguo, Zhang, Xiuping, Guan, Yue, Yang, Xudong, Li, Yang, Song, Gaochen, Guan, Lixin
Format: Article
Language:English
Subjects:
Apoptosis
bcl-2
blot分析
caspase-3
Disease
Gene expression
Ischemia
MicroRNAs
mRNA
Pathogenesis
Proteins
Studies
Surgery
Techniques and Methods: Emerging Technology in Neuroregeneration
Traumatic brain injury
大脑
小分子RNA
缺血/再灌注损伤
表达谱
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Summary:Rat models of focal cerebral ischemia/reperfusion injury were established by occlusion of the middle cerebral artery. Microarray analysis showed that 24 hours after cerebral ischemia, there were nine up-regulated and 27 down-regulated microRNA genes in cortical tissue. Bioinformatic analysis showed that bcl-2 was the target gene of microRNA-384-5p and microRNA-494, and caspase-3 was the target gene of microRNA-129, microRNA-320 and microRNA-326. Real-time PCR and western blot analyses showed that 24 hours after cerebral ischemia, bcl-2 mRNA and protein levels in brain tissue were significantly decreased, while caspase-3 mRNA and protein levels were significantly increased. This suggests that following cerebral ischemia, differentially expressed microRNA-384-5p, microRNA-494, microRNA-320, microRNA-129 and microRNA-326 can regulate bcl-2 and caspase-3 expression in brain tissue.
ISSN:1673-5374
1876-7958
DOI:10.3969/j.issn.1673-5374.2012.12.007