MicroRNA-26a inhibits proliferation by targeting high mobility group AT-hook 1 in breast cancer

To investigate the crucial role of miR-26a in breast cancer and to validate whether miR-26a could regulate proliferation of breast cancer cells by targeting high mobility group AT-hook 1 (HMGA1). Reverse transcription-polymerase chain reaction (RT-PCR) was used to quantify the expression levels of m...

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Published in:International journal of clinical and experimental pathology 2015, Vol.8 (1), p.368-373
Main Authors: Zhao, Xi-Xue, Yuan, Qing-Zhong, Mu, Dong-Po, Sun, Di-Wen, Bo, Qing-Ao, Pan, Guo-Zheng, Li, Guo-Qiang, Cui, Tao, Ding, Peng-Peng, You, Fa-Ping, Hao, Long, Wang, Ming-Xin, Zhang, Jian
Format: Article
Language:English
Subjects:
Blotting, Western
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cell Proliferation - genetics
Female
Gene Expression Regulation, Neoplastic - genetics
HMGA Proteins - biosynthesis
Humans
MicroRNAs - genetics
Original
Real-Time Polymerase Chain Reaction
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Summary:To investigate the crucial role of miR-26a in breast cancer and to validate whether miR-26a could regulate proliferation of breast cancer cells by targeting high mobility group AT-hook 1 (HMGA1). Reverse transcription-polymerase chain reaction (RT-PCR) was used to quantify the expression levels of miR-26a in breast cancer and adjacent non-cancerous breast tissues. MTT, cell migration and invasion assay were carried out to characterize the miR-26a function. Finally, to validate the target gene of miR-26a, luciferase reporter assay was employed, followed by RT-PCR and Western blot confirmation. Compared with normal tissues, a significant down-regulation of miR-26a expression was observed in breast cancer tissues (P=0.002). miR-26a suppresses MDA-MB-231 and Mcf-7 breast cancer cell lines proliferation and motility. The luciferase activity was significantly decreased after co-transfection with psiCHECK-2/HMGA1 3'-UTR and miR-26a mimics in comparison with control cells, and qRT-PCR and Western blotting analysis found that HMGA1 expression at the mRNA and protein levels decreased in the miR-26a mimic-treatment group relative to NC. MTT assay showed that down regulation of HMGA1 by siRNA could significantly enhance the tumor-suppressive effect of miR-26a (P < 0.05). The results of the present study indicate that miR-26a may be associated with human breast carcinogenesis, which inhibits tumor cell proliferation by targeting HMGA1.
ISSN:1936-2625