Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains

Abstract Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variet...

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Bibliographic Details
Published in:G3 : genes - genomes - genetics 2015-03, Vol.5 (3), p.361-370
Main Authors: Chen, Jun-Song, Beckley, Janel R, McDonald, Nathan A, Ren, Liping, Mangione, MariaSanta, Jang, Sylvia J, Elmore, Zachary C, Rachfall, Nicole, Feoktistova, Anna, Jones, Christine M, Willet, Alaina H, Guillen, Rodrigo, Bitton, Danny A, Bähler, Jürg, Jensen, Michael A, Rhind, Nick, Gould, Kathleen L
Format: Article
Language:English
Subjects:
Cell Division - genetics
DNA Damage - genetics
Gene Deletion
Genes, Fungal
Investigations
Schizosaccharomyces - cytology
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
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Summary:Abstract Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.
ISSN:2160-1836
2160-1836
DOI:10.1534/g3.114.015701