Proteomic Study of Microsomal Proteins Reveals a Key Role for Arabidopsis Annexin 1 in Mediating Heat Stress-Induced Increase in Intracellular Calcium Levels

To understand the early signaling steps in the response of plant cells to increased environmental temperature, 2-D difference gel electrophoresis was used to study the proteins in microsomes of Arabidopsis seedlings that are regulated early during heat stress. Using mass spectrometry, 19 microsomal...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2015-03, Vol.14 (3), p.686-694
Main Authors: Wang, Xu, Ma, Xiaolong, Wang, Hui, Li, Bingjie, Clark, Greg, Guo, Yi, Roux, Stan, Sun, Daye, Tang, Wenqiang
Format: Article
Language:English
Subjects:
Annexin A2 - genetics
Annexin A2 - metabolism
Annexins - genetics
Annexins - metabolism
Arabidopsis
Arabidopsis - physiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Calcium - metabolism
Cytoplasm - metabolism
Gene Expression Regulation, Plant
Heat-Shock Response
Hot Temperature
Microsomes - metabolism
Mutation
Proteomics - methods
Seedlings - metabolism
Up-Regulation
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Summary:To understand the early signaling steps in the response of plant cells to increased environmental temperature, 2-D difference gel electrophoresis was used to study the proteins in microsomes of Arabidopsis seedlings that are regulated early during heat stress. Using mass spectrometry, 19 microsomal proteins that showed an altered expression level within 5 min after heat treatment were identified. Among these proteins, annexin 1 (AtANN1) was one of those up-regulated rapidly after heat-shock treatment. Functional studies show loss-of-function mutants for AtANN1 and its close homolog AtANN2 were more sensitive to heat-shock treatment, whereas plants overexpressing AtANN1 showed more resistance to this treatment. Correspondingly, the heat-induced expression of heat-shock proteins and heat-shock factors is inhibited in ann1/ann2 double mutant, and the heat-activated increase in cytoplasmic calcium concentration ([Ca2+]cyt) is greatly impaired in the ann1 mutant and almost undetectable in ann1/ann2 double mutant. Taken together these results suggest that AtANN1 is important in regulating the heat-induced increase in [Ca2+]cyt and in the response of Arabidopsis seedlings to heat stress.
ISSN:1535-9476
1535-9484
DOI:10.1074/mcp.M114.042697