Protein expression of sensory and motor nerves

• Published in: Neural regeneration research 2012-02, Vol.7 (5), p.369-375
• Main Authors: Ren, Zhiwu, Wang, Yu, Peng, Jiang, Zhang, Li, Xu, Wenjing, Liang, Xiangdang, Zhao, Qing, Lu, Shibi
• Format: Article
• Language: English
• Subjects: Techniques and Methods: Spinal Cord Injury and Neuroregeneration; 串联质谱; 双向凝胶电泳; 周围神经损伤; 蛋白表达; 蛋白质表达; 谷胱甘肽过氧化物酶; 运动神经; 高效液相色谱法
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.05.008

The present study utilized samples from bilateral motor branches of the femoral nerve, as well as saphenous nerves, ventral roots, and dorsal roots of the spinal cord, to detect differential protein expression using two-dimensional gel electrophoresis and nano ultra-high performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry techniques. A mass spectrum was identified using the Mascot search. Results revealed differential expression of 11 proteins, including transgelin, Ig kappa chain precursor, plasma glutathione peroxidase precursor, an unnamed protein product （gil55628）, gfyceraldehyde-3-phosphate dehydrogenase-like protein, lactoylgfutathione lyase, adenyfate kinase isozyme 1, two unnamed proteins products （gil55628 and gi11334163）, and poly（rC）-binding protein 1 in motor and sensory nerves. Results suggested that these proteins played roles in specific nerve regeneration following peripheral nerve injury and served as specific markers for motor and sensory nerves.