7, 8-dihydroxycoumarin improves neurological Function in a mouse model of sciatic nerve injury

• Published in: Neural regeneration research 2012-02, Vol.7 (6), p.445-450
• Main Authors: Du, Jianshi, Zhao, Qing, Zhang, Yingli, Wang, Yu, Ma, Ming
• Format: Article
• Language: English
• Subjects: Drug dosages; Ischemia; Laboratory animals; Ostomy; Protein expression; Proteins; Research and Report: Traditional Chinese Medicine and Neuroregeneration; Spinal cord; 实时PCR; 小鼠模型; 检测结果; 生长相关蛋白; 神经传导速度; 神经功能; 神经损伤; 腹腔注射
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.06.007

In the present study, a mouse model of sciatic nerve injury was treated with intraperitoneal injection of 7, 8-dihydroxycoumarin （10, 5, or 2.5 mg/kg per day）. Western blot and real-time PCR results showed that growth associated protein 43 expression was significantly increased in the L4-6 segments of the spinal cord. The amplitude and velocity of motor nerve conduction in the sciatic nerve were significantly increased in model mice. In addition, the appearance of the myelin sheath in the injured sciatic nerve was regular, with an even thickness and clear outline, and the surrounding fibroplasia was not obvious. Our results indicate that 7, 8-dihydroxycoumarin can promote the repair of injured nerve by upregulating growth associated protein 43 expression in the corresponding spinal cord segments of mice with sciatic nerve injury.