Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two c...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2013-08, Vol.341 (6149), p.1009-1012
Main Authors: Bhogaraju, Sagar, Cajanek, Lukas, Fort, Cécile, Blisnick, Thierry, Weber, Kristina, Taschner, Michael, Mizuno, Naoko, Lamla, Stefan, Bastin, Philippe, Nigg, Erich A., Lorentzen, Esben
Format: Article
Language:English
Subjects:
Antennas
Antibodies
Binding sites
Biochemistry
Cell Line, Tumor
Cell walls
Cells
Chlamydomonas reinhardtii
Chlamydomonas reinhardtii - genetics
Chlamydomonas reinhardtii - metabolism
Cilia
Cilia - genetics
Cilia - physiology
Crystal structure
Crystallography, X-Ray
Cytokines
Cytoskeletal Proteins
Cytoskeletal Proteins - chemistry
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Disorders
Enzymes
Formations
Gene Knockdown Techniques
Humans
Integrals
Kidney diseases
Life Sciences
Machinery
Maintenance
Microbiology and Parasitology
Molecular biology
Muscle Proteins
Muscle Proteins - chemistry
Muscle Proteins - genetics
Muscle Proteins - metabolism
Plant Proteins
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Point Mutation
Protein Structure, Tertiary
Protein Transport
Proteins
RNA, Small Interfering
RNA, Small Interfering - genetics
Small interfering RNA
Stem cells
Thermophoresis
Transport
Tubulin
Tubulin - metabolism
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Summary:Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1240985