Different effects of sonoporation on cell morphology and viability

The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a...

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Bibliographic Details
Published in:Biomolecules & biomedicine 2012-05, Vol.12 (2), p.64-68
Main Authors: Zhang, Ji-Zhen, Saggar, Jasdeep K, Zhou, Zhao-Li, Hu, Bing
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell physiology
Cell Survival - physiology
Cells - ultrastructure
Culture Media
drug delivery
Fluoresceins - metabolism
Health aspects
Humans
low frequency ultrasound
Male
microbubble contrast agents
Microbubbles
Microscopy, Electron, Scanning
Microscopy, Fluorescence
molecular delivery
Morphology
Patient outcomes
Sonication
sonoporation
Ultrasonics
Ultrasonics in medicine
ultrasound
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Summary:The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a cell impermeant dye). We investigated changes in cell morphology immediately after sonoporation under scanning electron microscope (SEM) and changes in cell viability immediately and 6 h after sonoporation under fluorescence microscope. It was shown that various levels of intracellular calcein uptake and changes in cell morphology can be caused immediately after sonoporation: smooth cell surface, pores in the membrane and irregular cell surface. Immediately after sonoporation, both groups of cells with high levels of calcein uptake and low levels of calcein uptake were viable; 6 h after sonoporation, group of cells with low levels of calcein uptake still remained viable, while group of cells with high levels of calcein uptake died. Sonoporation induces different effects on cell morphology, intracellular calcein uptake and cell viability.
ISSN:1512-8601
2831-0896
1840-4812
2831-090X
DOI:10.17305/bjbms.2012.2497