Silver nanoparticle based antibacterial methacrylate hydrogels potential for bone graft applications

• Published in: Materials Science & Engineering C 2015-05, Vol.50, p.332-340
• Main Authors: González-Sánchez, M. Isabel, Perni, Stefano, Tommasi, Giacomo, Morris, Nathanael Glyn, Hawkins, Karl, López-Cabarcos, Enrique, Prokopovich, Polina
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Anti-Bacterial Agents - pharmacology; Antiinfectives and antibacterials; Bone Transplantation; Bones; Cell Death - drug effects; Cell Line; Diffusion; Elastic Modulus - drug effects; Grafting; Hydrogels; Hydrogels - pharmacology; Infections; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Methacrylates - pharmacology; Methicillin-resistant Staphylococcus aureus (MRSA); Methicillin-Resistant Staphylococcus aureus - drug effects; Mice; Microbial Sensitivity Tests; Nanoparticles; Osteoblasts - cytology; Osteoblasts - drug effects; Polymerization; Rheology - drug effects; Silver; Silver - chemistry; Silver - pharmacology; Spectrophotometry, Ultraviolet; Staphylococcus epidermidis; Staphylococcus epidermidis - drug effects; Surgical implants
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2015.02.002

Infections are frequent and very undesired occurrences after orthopedic procedures; furthermore, the growing concern caused by the rise in antibiotic resistance is progressively dwindling the efficacy of such drugs. Artificial bone graft materials could solve some of the problems associated with the gold standard use of natural bone graft such as limited bone material, pain at the donor site and rejections if donor tissue is used. We have previously described new acrylate base nanocomposite hydrogels as bone graft materials. In the present paper, we describe the integration of silver nanoparticles in the polymeric mineralized biomaterial to provide non-antibiotic antibacterial activity against Staphylococcus epidermidis and Methicillin-resistant Staphylococcus aureus. Two different crosslinking degrees were tested and the silver nanoparticles were integrated into the composite matrix by means of three different methods: entrapment in the polymeric hydrogel before the mineralization; diffusion during the process of calcium phosphate crystallization and adsorption post-mineralization. The latter being generally the most effective method of encapsulation; however, the adsorption of silver nanoparticles inside the pores of the biomaterial led to a decreasing antibacterial activity for adsorption time longer than 2days. •Acrylate based hydrogels were prepared.•Hydrogels were mineralized through reaction diffusion.•Silver nanoparticles were encapsulated in different ways.•Nanoparticle adsorption after mineralization was the most effective antibacterial method.