Protease activated receptor-1 mediates cytotoxicity during ischemia using in vivo and in vitro models

Highlights • PAR-1 knockdown neuronal and astrocytes cells are negative for TUNEL-positive cells. • PAR-1 knockdown results in 70% reduction of dying neurons with improved neurological deficits. • Knockdown or inhibition of PAR-1 would provide neuroprotection in the brain from ischemic injury.

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Bibliographic Details
Published in:Neuroscience 2014-12, Vol.281, p.229-240
Main Authors: Rajput, P.S, Lyden, P.D, Chen, B, Lamb, J.A, Pereira, B, Lamb, A, Zhao, L, Lei, I.-F, Bai, J
Format: Article
Language:English
Subjects:
Animals
Astrocytes - metabolism
Biological and medical sciences
Brain Ischemia - etiology
Brain Ischemia - metabolism
Brain Ischemia - prevention & control
Caudate Nucleus - metabolism
Cells, Cultured
cerebral ischemia
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Infarction, Middle Cerebral Artery - complications
Lentivirus
lentivirus knock-down
Male
Medical sciences
Mice
Mice, Knockout
Neurology
Neurons - metabolism
Neuroprotection
oxygen–glucose deprivation
protease activated receptor-1
Rats
Rats, Sprague-Dawley
Rats, Transgenic
Receptor, PAR-1 - metabolism
thrombin
Thrombin - metabolism
Vascular diseases and vascular malformations of the nervous system
Vertebrates: nervous system and sense organs
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Description
Summary:Highlights • PAR-1 knockdown neuronal and astrocytes cells are negative for TUNEL-positive cells. • PAR-1 knockdown results in 70% reduction of dying neurons with improved neurological deficits. • Knockdown or inhibition of PAR-1 would provide neuroprotection in the brain from ischemic injury.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2014.09.038