The Viability and Protein Expression of Beijing/W Lineage Mycobacterium tuberculosis Circulating in Xinjiang, China

Beijing/W lineage strains of Mycobacterium tuberculosis spread faster than other strains, tend to be more virulent and frequently associated with drug resistance. In this study, to distinguish the characteristics of Beijing/W lineage and non-Beijing/W lineage M. tuberculosis , we assessed the growth...

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Bibliographic Details
Published in:Current microbiology 2015-05, Vol.70 (5), p.735-744
Main Authors: Li, Feng, Li, Hua, Zuo, Wei-ze, Mi, Ligu, Wang, Xian, Wang, Yuanzhi, Wang, Hong, Shen, Aiping, Cao, Shuaili, Yuan, Li
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Biomedical and Life Sciences
Biotechnology
Blotting, Western
Cell Line
China
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Drug resistance
Gene Expression Profiling
Hypoxia
Life Sciences
Macrophages - microbiology
Mice
Microbial Viability
Microbiology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - chemistry
Mycobacterium tuberculosis - isolation & purification
Mycobacterium tuberculosis - physiology
Proteins
Real-Time Polymerase Chain Reaction
RNA, Bacterial - analysis
RNA, Bacterial - genetics
Sequence Analysis, DNA
Tuberculosis
Tuberculosis - microbiology
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Summary:Beijing/W lineage strains of Mycobacterium tuberculosis spread faster than other strains, tend to be more virulent and frequently associated with drug resistance. In this study, to distinguish the characteristics of Beijing/W lineage and non-Beijing/W lineage M. tuberculosis , we assessed the growth between the two groups under conditions of hypoxia, nutrient starvation, and intracellular growth in murine macrophages. We also examined the DNA, RNA, and protein levels of 5 major M. tuberculosis proteins, including HspX, Hsp65, 38 kDa, Ag85B, and MPT64 of the different types of strains by sequencing, quantitative RT-PCR, and Western blotting. The results showed that Beijing/W and non-Beijing/W lineage strains of M. tuberculosis have similar viability in ex vivo culture but differ in their ability to survive within macrophages, and the intracellular viability of the Beijing/W lineage strains was significantly more than the viability of the non-Beijing/W lineage strains at 2, 3, and 5 days after infection ( P  
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-015-0776-z