Comparative evaluation of the protective efficacy of two formulations of a recombinant Chlamydia abortus subunit candidate vaccine in a mouse model

Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the D...

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Bibliographic Details
Published in:Vaccine 2015-04, Vol.33 (15), p.1865-1872
Main Authors: Pan, Qing, Pais, Roshan, Ohandjo, Adaugo, He, Cheng, He, Qing, Omosun, Yusuf, Igietseme, J.U, Eko, F.O
Format: Article
Language:English
Subjects:
Abortion
Adjuvants, Immunologic
Administration, Intranasal
Allergy and Immunology
Animal diseases
Animals
Antibodies, Bacterial - immunology
Bacteria
Bacterial Vaccines - immunology
Chlamydia
Chlamydia - immunology
Chlamydia abortus
Chlamydia Infections - immunology
Chlamydia Infections - microbiology
Chlamydia Infections - prevention & control
Cloning
Cross Protection
Cytokines
Dendritic cells
Dendritic Cells - immunology
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Immunity
Immunity, Innate
Immunization
Infections
Kinases
Ligands
Mice
Mice, Inbred C57BL
Oligodeoxyribonucleotides - immunology
Pmp18D
Proteins
Sexually transmitted diseases
STD
Studies
T-Lymphocytes - immunology
Toll-Like Receptors - immunology
Vaccine delivery
Vaccines
Vaccines, Subunit - administration & dosage
Vaccines, Subunit - immunology
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
Vagina - microbiology
Vibrio cholerae
Vibrio cholerae - immunology
Vibrio cholerae - isolation & purification
Zoonoses
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Summary:Highlights • The ability of recombinant C. abortus Pmp18D formulated in CpG + FL adjuvants or Vibrio cholerae ghosts (VCGs) to induce innate and cross protective immunity against genital C. abortus infection was evaluated. • VCG-delivered rPmp18D was more effective than CpG + FL in stimulating the DC expression of molecules critically involved in T cell activation and differentiation, and activation of innate immune signaling involving TLRs and NLRP3 inflammasome. • VCG-delivered rPmp18D elicited more robust antigen-specific cellular and humoral immune responses in mice compared to CpG + FL. • Vaccine delivery with VCG induced superior cross-protective immunity than delivery with a combination of CpG1826 and FL. • The vaccine delivery and immunomodulatory capacity of VCG is thus superior to that of CpG + FL adjuvants.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2015.02.007