Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative d...

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Published in:Iranian journal of basic medical sciences 2014-12, Vol.17 (12), p.986-992
Main Authors: Havakhah, Shahrzad, Sadeghnia, Hamid R, Hajzadeh, Mosa-Al-Reza, Roshan, Nama Mohammadian, Shafiee, Somayeh, Hosseinzadeh, Hossein, Mohareri, Narges, Rad, Abolfazl Khajavi
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Language:English
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Summary:There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI. IRI was induced by clamping of bilateral renal arteries for 40 min fallowed by reperfusion for 180 min. NSE was prepared in a Soxhlet extractor and administrated with doses of 150 mg/kg or 300 mg/kg at 1 hr before ischemia induction (P-150 and 300) or at the beginning of reperfusion phase (T-150 and 300), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination. The kidneys of untreated IRI rats had a higher histopathological score (P
ISSN:2008-3866
2008-3874