Expression profile of developmentally important genes between hand-made cloned buffalo embryos produced from reprogramming of donor cell with oocytes extract and selection of recipient cytoplast through brilliant cresyl blue staining and in vitro fertilized embryos

Purpose To compare the expression profile of developmentally important genes between hand-made cloned buffalo embryos produced from reprogramming of donor cell with oocyte extracts and selection of recipient cytoplast through brilliant cresyl blue staining and in vitro fertilized (IVF) embryos. Meth...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2014-11, Vol.31 (11), p.1541-1552
Main Authors: EM, Sadeesh, Kataria, Meena, S, Balhara, Yadav, PS
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Buffaloes - embryology
Buffaloes - genetics
Cell culture
Cloning
Cloning, Organism - veterinary
Cytoplasm - metabolism
DNA methylation
Embryo Biology
Embryo, Mammalian - cytology
Embryo, Mammalian - metabolism
Embryos
Epigenetics
Fertilization in Vitro - veterinary
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genetic engineering
Gynecology
Human Genetics
In vitro fertilization
Medicine
Medicine & Public Health
Nuclear Transfer Techniques - veterinary
Oocytes - metabolism
Oxazines
Reproductive Medicine
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Summary:Purpose To compare the expression profile of developmentally important genes between hand-made cloned buffalo embryos produced from reprogramming of donor cell with oocyte extracts and selection of recipient cytoplast through brilliant cresyl blue staining and in vitro fertilized (IVF) embryos. Methods Hand-made cloned embryos were produced using oocyte extracts treated donor cells and brilliant cresyl blue (BCB) stained recipient cytoplasts. IVF embryos were produced by culturing 15–20 COCs in BO capacitated sperms from frozen thawed buffalo semen and the mRNA expression patterns of genes implicated in metabolism ( GLUT1 ), pluripotency ( OCT4 ), DNA methylation ( DNMT1 ), pro- apoptosis ( BAX ) and anti-apoptosis ( BCL2 ) were evaluated at 8- to16- cell stage embryos. Results A significantly ( P  
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-014-0316-y