Successful live birth from oocytes after more than 14 years of cryopreservation

Objective To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium. Design Clinical application. Setting University Affiliated, Private IVF center. Patient A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2014-11, Vol.31 (11), p.1553-1555
Main Authors: Urquiza, María Fernanda, Carretero, Inés, Cano Carabajal, Paola Rita, Pasqualini, Rodolfo Agustín, Felici, María Macarena, Pasqualini, Rodolfo Sergio, Quintans, Carlos José
Format: Article
Language:English
Subjects:
Adult
Case reports
Cryopreservation
Cryopreservation - methods
Embryos
Female
Fertility
Fertility Preservation
Fertility Preservation - methods
Gynecology
Human Genetics
Humans
In vitro fertilization
Infertility
Live Birth
Medicine
Medicine & Public Health
Oocytes - cytology
Oocytes - growth & development
Pregnancy
Reproductive Medicine
Sodium
Sperm
Sperm Injections, Intracytoplasmic
Time Factors
Ultrasonic imaging
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Summary:Objective To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium. Design Clinical application. Setting University Affiliated, Private IVF center. Patient A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved by slow freezing in a low-sodium medium 14 years and 6 months before, when she was 24 years old. Main outcome measure(s) Survival, fertilization, cleavage, clinical pregnancy and delivery. Result(s) From six metaphase-II oocytes thawed, two survived, one was fertilized after ICSI and a cleaving embryo was transferred on day 3. A single term pregnancy was achieved, ending with the delivery of a healthy girl. Conclusion(s) Cryopreservation after slow freezing in a sodium depleted medium maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. As far as is known, this case represents, up to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-014-0318-9