Global multilocus sequence typing analysis of Mycoplasma bovis isolates reveals two main population clusters

Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To...

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Bibliographic Details
Published in:Journal of clinical microbiology 2015-03, Vol.53 (3), p.789-794
Main Authors: Rosales, R S, Churchward, C P, Schnee, C, Sachse, K, Lysnyansky, I, Catania, S, Iob, L, Ayling, R D, Nicholas, R A J
Format: Article
Language:English
Subjects:
Animals
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - microbiology
Clinical Veterinary Microbiology
Cluster Analysis
Genes, Bacterial
Genes, Essential
Genetic Variation
Genotype
Global Health
Molecular Epidemiology
Molecular Sequence Data
Multilocus Sequence Typing
Mycoplasma bovis
Mycoplasma bovis - classification
Mycoplasma bovis - genetics
Mycoplasma bovis - isolation & purification
Mycoplasma Infections - epidemiology
Mycoplasma Infections - microbiology
Mycoplasma Infections - veterinary
Phylogeography
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Summary:Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better understanding of the genetic diversity of this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the characterization of 137 M. bovis isolates from diverse geographical origins, obtained from healthy or clinically infected cattle. After in silico analysis, a final set of 7 housekeeping genes was selected (dnaA, metS, recA, tufA, atpA, rpoD, and tkt). MLST analysis demonstrated the presence of 35 different sequence types (STs) distributed in two main clonal complexes (CCs), defined at the double-locus variant level, namely, CC1, which included most of the British and German isolates, and CC2, which was a more heterogeneous and geographically distant group of isolates, including European, Asian, and Australian samples. Index of association analysis confirmed the clonal nature of the investigated M. bovis population, based on MLST data. This scheme has demonstrated high discriminatory power, with the analysis showing the presence of genetically distant and divergent clusters of isolates predominantly associated with geographical origins.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01910-14