Single-molecule arrays for ultrasensitive detection of host immune response to dengue virus infection

Dengue virus serotype 2-positive plasma was applied to two indirect single-molecule arrays (Simoas) for the detection of anti-dengue virus IgG and IgM. The Simoas were 1,000 and 10,000 times more sensitive than enzyme-linked immunosorbent assays (ELISAs) for the detection of IgG and IgM, respectivel...

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Bibliographic Details
Published in:Journal of clinical microbiology 2015-05, Vol.53 (5), p.1722-1724
Main Authors: Gaylord, Shonda T, Abdul-Aziz, Sama, Walt, David R
Format: Article
Language:English
Subjects:
Antibodies, Viral - blood
Clinical Laboratory Techniques - methods
Dengue - diagnosis
Dengue - immunology
Dengue virus
Dengue Virus - immunology
Diagnostic Tests, Routine - methods
Humans
Immunoassays
Immunoglobulin G - blood
Immunoglobulin M - blood
Protein Array Analysis - methods
Sensitivity and Specificity
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Summary:Dengue virus serotype 2-positive plasma was applied to two indirect single-molecule arrays (Simoas) for the detection of anti-dengue virus IgG and IgM. The Simoas were 1,000 and 10,000 times more sensitive than enzyme-linked immunosorbent assays (ELISAs) for the detection of IgG and IgM, respectively. Using Simoas, serology may be used for the detection of dengue virus infection in the acute phase.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.03487-14