Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition

The alcohol dehydrogenase promoter P adhE and mixed acid fermentation pathway deficient mutants of Escherichia coli were employed to produce poly(3-hydroxybutyrate) (P3HB) under microaerobic condition. The E. coli mutant with ackA-pta, poxB, ldhA, and adhE deletions accumulated 0.67 g/L P3HB, up to...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-5
Main Authors: Liang, Jian, Hou, Xue, Zheng, Wei-Tao, Wei, Xiao-Xing, Li, Zheng-Jun
Format: Article
Language:English
Subjects:
Acids
Aerobiosis
Agricultural production
Biosynthesis
Carbon
Cell growth
Dehydrogenases
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins - biosynthesis
Escherichia coli Proteins - genetics
Ethanol
Fermentation
Gene mutations
Genetic aspects
Glucose
Glucose - metabolism
Glycerol
Hydroxybutyrates - metabolism
Laboratories
Metabolic Engineering
Metabolism
Observations
Plasmids
Plasmids - genetics
Polyesters - metabolism
Polyhydroxybutyrate
Properties
Raw materials
Studies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The alcohol dehydrogenase promoter P adhE and mixed acid fermentation pathway deficient mutants of Escherichia coli were employed to produce poly(3-hydroxybutyrate) (P3HB) under microaerobic condition. The E. coli mutant with ackA-pta, poxB, ldhA, and adhE deletions accumulated 0.67 g/L P3HB, up to 78.84% of cell dry weight in tube cultivation. The deletion of pyruvate formate-lyase gene pflB drastically decreased P3HB production and P3HB content to 0.09 g/L and 24.44%, respectively. Overexpressing pflB via the plasmid in its knocked out mutant restored cell growth and P3HB accumulation, indicating the importance of the pyruvate formate-lyase in microaerobic carbon metabolism. The engineered E. coli BWapld (pWYC09) produced 5.00 g/L P3HB from 16.50 g/L glucose in 24 h batch fermentation, and P3HB production yield from glucose was 0.30 g/g, which reached up to 63% of maximal theoretical yield.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/789315