Voltage controlled nano-injection system for single-cell surgery

Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses...

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Bibliographic Details
Published in:Nanoscale 2012-01, Vol.4 (19), p.5843-5846
Main Authors: Adam Seger, R, Actis, Paolo, Penfold, Catherine, Maalouf, Michelle, Vilozny, Boaz, Pourmand, Nader
Format: Article
Language:English
Subjects:
Cell Line
Cell Membrane - metabolism
Electric potential
Electricity
Fibroblasts - metabolism
Fluorescent dyes
Fluorescent Dyes - chemistry
Fluorescent Dyes - metabolism
Humans
Microscopy, Electron, Scanning
Nanocomposites
Nanomaterials
Nanostructure
Nanotechnology - instrumentation
Platforms
Surgery
Voltage
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Description
Summary:Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes.
ISSN:2040-3364
2040-3372
DOI:10.1039/c2nr31700a