Transcriptome analysis of the effect of Vibrio alginolyticus infection on the innate immunity-related complement pathway in Epinephelus coioides

Orange-spotted grouper (Epinephelus coioides) with protogynous hermaphroditic features are one of the most economically important aquaculture species in Taiwan. However, larvae stage grouper are susceptible to infection by the bacterial pathogen Vibrio alginolyticus. To better understand the molecul...

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Bibliographic Details
Published in:BMC genomics 2014-12, Vol.15 (1), p.1102-1102, Article 1102
Main Authors: Wang, Yi-Da, Huang, Shin-Jie, Chou, Hong-Nong, Liao, Wen-Liang, Gong, Hong-Yi, Chen, Jyh-Yih
Format: Article
Language:English
Subjects:
Analysis
Animals
Antimicrobial Cationic Peptides - genetics
Antimicrobial Cationic Peptides - metabolism
Aquaculture
Biology
Complement Activation - genetics
Complement System Proteins - immunology
Computational Biology
Disease susceptibility
Encyclopedias
Epinephelus coioides
Experiments
Fish
Fish Diseases - genetics
Fish Diseases - immunology
Fish Diseases - metabolism
Fish Diseases - microbiology
Gene Expression
Gene Expression Profiling
Genes
Genetic aspects
Genomes
Genomics
Health aspects
High-Throughput Nucleotide Sequencing
Host-Pathogen Interactions - genetics
Host-Pathogen Interactions - immunology
Immune response
Immunity, Innate - genetics
Infections
Marine
Medical research
Medicine, Experimental
Metabolic Networks and Pathways
Metabolism
Molecular Sequence Annotation
Ontology
Phagocytosis - genetics
Phagocytosis - immunology
Signal Transduction
Staphylococcal infections
Staphylococcus aureus
Transcriptome
Vibrio alginolyticus
Vibrio alginolyticus - immunology
Vibrio Infections - veterinary
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Summary:Orange-spotted grouper (Epinephelus coioides) with protogynous hermaphroditic features are one of the most economically important aquaculture species in Taiwan. However, larvae stage grouper are susceptible to infection by the bacterial pathogen Vibrio alginolyticus. To better understand the molecular mechanisms of the immune response to V. alginolyticus in Epinephelus coioides larvae, we used high-throughput deep sequencing technology to study the effect of infection on gene expression. A total of 114,851,002 reads were assembled, consisting of 9,687,355,560 nucleotides; these were further assembled into 209,082 contigs with a mean length of 372 bp. Gene ontology (GO) analysis of the transcriptome revealed 12 cellular component subcategories, 16 molecular function subcategories, and 42 biological process subcategories (P value
ISSN:1471-2164
1471-2164
DOI:10.1186/1471-2164-15-1102