Fluid transfer in the everted human gallbladder

The rates of fluid transfer across human gallbladders obtained at cholecystectomy for cholelithiasis were determined by the measurement of weight changes of everted preparations under controlled conditions. Active transport of fluid from the mucosal to the serosal surface was indicated since weight...

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Bibliographic Details
Published in:The Journal of clinical investigation 1967-04, Vol.46 (4), p.606-614
Main Authors: Onstad, G R, Schoenfield, L J, Higgins, J A
Format: Article
Language:English
Subjects:
Biological Transport, Active
Body Weight
Chemical Phenomena
Chemistry, Physical
Chlorides - analysis
Cholecystokinin - pharmacology
Electrophysiology
Gallbladder - drug effects
Gallbladder - pathology
Gallbladder - physiology
Humans
Membrane Potentials
Mucous Membrane - physiology
Muscle Contraction
Norepinephrine - pharmacology
Osmosis
Potassium - analysis
Serous Membrane - physiology
Sodium - analysis
Temperature
Water-Electrolyte Balance
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Summary:The rates of fluid transfer across human gallbladders obtained at cholecystectomy for cholelithiasis were determined by the measurement of weight changes of everted preparations under controlled conditions. Active transport of fluid from the mucosal to the serosal surface was indicated since weight gain occurred with the same solution on both sides of the membrane and against hydrostatic, osmotic, and potential differences. With respect to sodium, the fluid transferred was isotonic to the bathing solutions. Metabolic inhibitors and temperature extremes inhibited weight gain. In addition, muscle contractions in this in vitro preparation were related to the rates and direction of fluid movement. Cholecystokinin increased muscle activity and caused weight loss in preparations that previously had gained weight. Norepinephrine caused weight gain or increased weight gain in all preparations tested. The direction of net fluid movement in the isolated everted human gallbladder was determined by the opposing forces of active mucosal transport and a filtration pressure generated by muscle contractions.
ISSN:0021-9738
DOI:10.1172/JCI105562