Cationic nanoemulsions as potential carriers for intracellular delivery

Successful cytosolic delivery enables opportunities for improved treatment of various genetic disorders, infectious diseases and cancer. Cationic nanoemulsions were designed using alternative excipients and evaluated for particle size, charge, effect of sterilization on its stability, DNA condensati...

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Bibliographic Details
Published in:Saudi pharmaceutical journal 2015-04, Vol.23 (2), p.188-194
Main Authors: Khachane, P.V., Jain, A.S., Dhawan, V.V., Joshi, G.V., Date, A.A., Mulherkar, R., Nagarsenker, M.S.
Format: Article
Language:English
Subjects:
Cationic
Cytosolic delivery
Didodecyldimethylammonium bromide
Intracellular delivery
Nanoemulsion
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Summary:Successful cytosolic delivery enables opportunities for improved treatment of various genetic disorders, infectious diseases and cancer. Cationic nanoemulsions were designed using alternative excipients and evaluated for particle size, charge, effect of sterilization on its stability, DNA condensation potential and cellular uptake efficiency. Various concentrations of non-ionic and ionic stabilizers were evaluated to design formula for colloidally stable cationic nanoemulsion. The nanoemulsion comprised of 5% Capmul MCM, 0.5% didodecyldimethylammonium bromide (DDAB), 1% phospholipid, 1% Poloxamer 188 and 2.25% glycerol and possessed particle size of 81.6±3.56nm and 137.1±1.57nm before and after steam sterilization, respectively. DNA condensation studies were carried out at various nanoemulsion: DNA ratios ranging from 1:1 to 10:1. Cell uptake studies were conducted on human embryonic kidney (HEK) cell lines which are widely reported for transfection studies. The nanoemulsions showed excellent cellular uptake as evaluated by fluorescence microscopy and flow cytometry. Overall, a colloidally stable cationic nanoemulsion with good DNA condensation ability was successfully fabricated for efficient cytosolic delivery and potential for in vivo effectiveness.
ISSN:1319-0164
2213-7475
DOI:10.1016/j.jsps.2014.07.007