Single Cell Wall Nonlinear Mechanics Revealed by a Multiscale Analysis of AFM Force-Indentation Curves

Individual plant cells are rather complex mechanical objects. Despite the fact that their wall mechanical strength may be weakened by comparison with their original tissue template, they nevertheless retain some generic properties of the mother tissue, namely the viscoelasticity and the shape of the...

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Bibliographic Details
Published in:Biophysical journal 2015-05, Vol.108 (9), p.2235-2248
Main Authors: Digiuni, Simona, Berne-Dedieu, Annik, Martinez-Torres, Cristina, Szecsi, Judit, Bendahmane, Mohammed, Arneodo, Alain, Argoul, Françoise
Format: Article
Language:English
Subjects:
Arabidopsis - cytology
Biomechanical Phenomena
Cell Wall - chemistry
Cells
Elasticity
Flowers & plants
Life Sciences
Mechanical properties
Microscopy, Atomic Force
Molecular Machines, Motors, and Nanoscale Biophysics
Pressure
Tissues
Viscosity
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Summary:Individual plant cells are rather complex mechanical objects. Despite the fact that their wall mechanical strength may be weakened by comparison with their original tissue template, they nevertheless retain some generic properties of the mother tissue, namely the viscoelasticity and the shape of their walls, which are driven by their internal hydrostatic turgor pressure. This viscoelastic behavior, which affects the power-law response of these cells when indented by an atomic force cantilever with a pyramidal tip, is also very sensitive to the culture media. To our knowledge, we develop here an original analyzing method, based on a multiscale decomposition of force-indentation curves, that reveals and quantifies for the first time the nonlinearity of the mechanical response of living single plant cells upon mechanical deformation. Further comparing the nonlinear strain responses of these isolated cells in three different media, we reveal an alteration of their linear bending elastic regime in both hyper- and hypotonic conditions.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2015.02.024