Structural organization of the Helicoverpa zea gene encoding the precursor protein for pheromone biosynthesis-activating neuropeptide and other neuropeptides

Sex pheromone biosynthesis in a number of moth species is induced by a conserved 33-amino acid amidated neuropeptide PBAN (pheromone biosynthesis-activating neuropeptide). We have isolated and characterized the Helicoverpa zea PBAN cDNA corresponding to a 766-nucleotide mRNA that is expressed in the...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1994-07, Vol.91 (14), p.6506-6510
Main Authors: Ma, P.W.K, Knipple, D.C, Roelofs, W.L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
ARN MENSAJERO
ARN MESSAGER
Base Sequence
Biology
BIOSINTESIS
BIOSYNTHESE
Blotting, Northern
Bombyx - genetics
Bombyx - metabolism
Butterflies & moths
Complementary DNA
Deoxyribonucleic acid
DNA
DNA - genetics
DNA Primers
DNA, Complementary - analysis
Exons
Female
FEROMONAS
Ganglia
GANGLION NERVEUX
GANGLIOS
GENE
GENES
Genomics
HELICOVERPA ZEA
Introns
Lepidoptera
Lepidoptera - genetics
Lepidoptera - metabolism
Male
Molecular Sequence Data
Neurology
Neuropeptides
Neuropeptides - biosynthesis
Open reading frames
PEPTIDE
PEPTIDOS
PHEROMONE
Polymerase Chain Reaction
Protein Precursors - biosynthesis
Proteins
RNA
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
Sex Attractants - biosynthesis
SEXE
SEXO
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Summary:Sex pheromone biosynthesis in a number of moth species is induced by a conserved 33-amino acid amidated neuropeptide PBAN (pheromone biosynthesis-activating neuropeptide). We have isolated and characterized the Helicoverpa zea PBAN cDNA corresponding to a 766-nucleotide mRNA that is expressed in the subesophageal ganglion of adult moths. This mRNA is encoded on a transcription unit comprising 6 exons. The longest open reading frame of the cDNA encodes a 194-amino acid precursor protein that contains the PBAN peptide sequence. Proteolytic processing of this protein, which has structural features consistent with its being a preprohormone, is predicted to generate Hez-PBAN and four additional neuropeptides having a common C-terminal pentapeptide motif, Phe-Xaa-Pro-(Arg or Lys)-Leu (Xaa = Gly, Ser, or Thr), which is also found in insect pyrokinin and myotropin peptide families.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.14.6506